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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Regulatory protein DegR (degR) CSB-YP303077BRJ
CSB-EP303077BRJ
CSB-BP303077BRJ
CSB-MP303077BRJ
CSB-EP303077BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Endonuclease V (nfi) CSB-YP303078EOD
CSB-EP303078EOD
CSB-BP303078EOD
CSB-MP303078EOD
CSB-EP303078EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ornithine carbamoyltransferase 2, phaseolotoxin-insensitive (argK) CSB-YP303079FGN
CSB-EP303079FGN
CSB-BP303079FGN
CSB-MP303079FGN
CSB-EP303079FGN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lilium superbum Maturase K (matK), partial CSB-YP303080LFT
CSB-EP303080LFT
CSB-BP303080LFT
CSB-MP303080LFT
CSB-EP303080LFT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Hemagglutinin (HA) CSB-YP303081IJA
CSB-EP303081IJA
CSB-BP303081IJA
CSB-MP303081IJA
CSB-EP303081IJA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus IAP-like protein p27 (p27) CSB-YP303082AEF
CSB-EP303082AEF
CSB-BP303082AEF
CSB-MP303082AEF
CSB-EP303082AEF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor P (efp) CSB-YP303083SMU
CSB-EP303083SMU
CSB-BP303083SMU
CSB-MP303083SMU
CSB-EP303083SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Galactose-6-phosphate isomerase subunit lacB (lacB) CSB-YP303084SUL
CSB-EP303084SUL
CSB-BP303084SUL
CSB-MP303084SUL
CSB-EP303084SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP303085SKX
CSB-EP303085SKX
CSB-BP303085SKX
CSB-MP303085SKX
CSB-EP303085SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Holliday junction resolvase recU (recU) CSB-YP303086SKX
CSB-EP303086SKX
CSB-BP303086SKX
CSB-MP303086SKX
CSB-EP303086SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flock house virus Protein B1 (B1) CSB-YP303087FCA
CSB-EP303087FCA
CSB-BP303087FCA
CSB-MP303087FCA
CSB-EP303087FCA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP303089SKY
CSB-EP303089SKY
CSB-BP303089SKY
CSB-MP303089SKY
CSB-EP303089SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA replication and repair protein recF (recF) CSB-YP303090SKY
CSB-EP303090SKY
CSB-BP303090SKY
CSB-MP303090SKY
CSB-EP303090SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus D-alanine--poly (phosphoribitol) ligase subunit 1 CSB-YP303091SUL
CSB-EP303091SUL
CSB-BP303091SUL
CSB-MP303091SUL
CSB-EP303091SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein grpE (grpE) CSB-YP303092SMT
CSB-EP303092SMT
CSB-BP303092SMT
CSB-MP303092SMT
CSB-EP303092SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HPr kinase/phosphorylase (hprK) CSB-YP303093SMT
CSB-EP303093SMT
CSB-BP303093SMT
CSB-MP303093SMT
CSB-EP303093SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Mb2921c (Mb2921c), partial CSB-YP303094MVH
CSB-EP303094MVH
CSB-BP303094MVH
CSB-MP303094MVH
CSB-EP303094MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0719 transmembrane protein Mb2631 (Mb2631), partial CSB-YP303095MVH1
CSB-EP303095MVH1
CSB-BP303095MVH1
CSB-MP303095MVH1
CSB-EP303095MVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein rexA (rexA) CSB-YP303096BDJ
CSB-EP303096BDJ
CSB-BP303096BDJ
CSB-MP303096BDJ
CSB-EP303096BDJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage phi15 Early protein GP16.5 (16.5) CSB-YP303097BBB
CSB-EP303097BBB
CSB-BP303097BBB
CSB-MP303097BBB
CSB-EP303097BBB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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