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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE (arnE), partial CSB-YP305628SWW1
CSB-EP305628SWW1
CSB-BP305628SWW1
CSB-MP305628SWW1
CSB-EP305628SWW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster cAMP-dependent protein kinase type II regulatory subunit (Pka-R2) CSB-YP305629DLU
CSB-EP305629DLU
CSB-BP305629DLU
CSB-MP305629DLU
CSB-EP305629DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Odorant receptor 23a (Or23a), partial CSB-YP305630DLU1
CSB-EP305630DLU1
CSB-BP305630DLU1
CSB-MP305630DLU1
CSB-EP305630DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Odorant receptor 33b (Or33b), partial CSB-YP305631DLU1
CSB-EP305631DLU1
CSB-BP305631DLU1
CSB-MP305631DLU1
CSB-EP305631DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Odorant receptor 47b (Or47b), partial CSB-YP305632DLU1
CSB-EP305632DLU1
CSB-BP305632DLU1
CSB-MP305632DLU1
CSB-EP305632DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster RPII140-upstream gene protein (140up), partial CSB-YP305633DLU1
CSB-EP305633DLU1
CSB-BP305633DLU1
CSB-MP305633DLU1
CSB-EP305633DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dendroaspis angusticeps Thrombostatin CSB-YP305634DBG
CSB-EP305634DBG
CSB-BP305634DBG
CSB-MP305634DBG
CSB-EP305634DBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chrysanthemum coronarium Thaumatin-like protein 5 CSB-YP305635CKC
CSB-EP305635CKC
CSB-BP305635CKC
CSB-MP305635CKC
CSB-EP305635CKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspidelaps scutatus Ascarin CSB-YP305636APV
CSB-EP305636APV
CSB-BP305636APV
CSB-MP305636APV
CSB-EP305636APV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta vulgaris Defensin-like protein AX2 CSB-YP305637BSQ
CSB-EP305637BSQ
CSB-BP305637BSQ
CSB-MP305637BSQ
CSB-EP305637BSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Goat Cathelicidin-2 (CATHL2) CSB-YP305638GO
CSB-EP305638GO
CSB-BP305638GO
CSB-MP305638GO
CSB-EP305638GO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria infrafrenata Frenatin-2 CSB-YP305639LHN
CSB-EP305639LHN
CSB-BP305639LHN
CSB-MP305639LHN
CSB-EP305639LHN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloydius blomhoffii Thrombin-like enzyme halystase CSB-YP305640GGN
CSB-EP305640GGN
CSB-BP305640GGN
CSB-MP305640GGN
CSB-EP305640GGN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans Periplasmic nitrate reductase (napA), partial CSB-YP305641DJN
CSB-EP305641DJN
CSB-BP305641DJN
CSB-MP305641DJN
CSB-EP305641DJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syncephalastrum racemosum Syncephapepsin (SPSR) CSB-YP305642FPW
CSB-EP305642FPW
CSB-BP305642FPW
CSB-MP305642FPW
CSB-EP305642FPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermoautotrophicus 5,10-methenyltetrahydromethanopterin hydrogenase (hmd) CSB-YP305643MFE
CSB-EP305643MFE
CSB-BP305643MFE
CSB-MP305643MFE
CSB-EP305643MFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0785.1 (MJ0785.1), partial CSB-YP305644MRU1
CSB-EP305644MRU1
CSB-BP305644MRU1
CSB-MP305644MRU1
CSB-EP305644MRU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor 1-alpha CSB-YP305645MGF
CSB-EP305645MGF
CSB-BP305645MGF
CSB-MP305645MGF
CSB-EP305645MGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides forsythus 60 kDa chaperonin (groL), partial CSB-YP305646BOO
CSB-EP305646BOO
CSB-BP305646BOO
CSB-MP305646BOO
CSB-EP305646BOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Isopropylmalate/citramalate isomerase large subunit (leuC) CSB-YP305647MRU
CSB-EP305647MRU
CSB-BP305647MRU
CSB-MP305647MRU
CSB-EP305647MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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