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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Horse Dander allergen Equ c 2.0102 CSB-YP305568HO
CSB-EP305568HO
CSB-BP305568HO
CSB-MP305568HO
CSB-EP305568HO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ1479.1 (MJ1479.1) CSB-YP305569MRU
CSB-EP305569MRU
CSB-BP305569MRU
CSB-MP305569MRU
CSB-EP305569MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0210.1 (MJ0210.1), partial CSB-YP305570MRU1
CSB-EP305570MRU1
CSB-BP305570MRU1
CSB-MP305570MRU1
CSB-EP305570MRU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria chloris Caerin-1.9 CSB-YP305571LHG
CSB-EP305571LHG
CSB-BP305571LHG
CSB-MP305571LHG
CSB-EP305571LHG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized polyferredoxin-like protein MJ0514.1 (MJ0514.1) CSB-YP305572MRU
CSB-EP305572MRU
CSB-BP305572MRU
CSB-MP305572MRU
CSB-EP305572MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Staphopain A (sspP) CSB-YP305573FKZ
CSB-BP305573FKZ
CSB-MP305573FKZ
CSB-EP305573FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0526.1 (MJ0526.1) CSB-YP305574MRU
CSB-EP305574MRU
CSB-BP305574MRU
CSB-MP305574MRU
CSB-EP305574MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Putative transposase MJ0856.1 (MJ0856.1) CSB-YP305575MRU
CSB-EP305575MRU
CSB-BP305575MRU
CSB-MP305575MRU
CSB-EP305575MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Putative type-2 restriction enzyme MjaVIP (mjaVIRP) CSB-YP305576MRU
CSB-EP305576MRU
CSB-BP305576MRU
CSB-MP305576MRU
CSB-EP305576MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ1292.1 (MJ1292.1) CSB-YP305577MRU
CSB-EP305577MRU
CSB-BP305577MRU
CSB-MP305577MRU
CSB-EP305577MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaK (dnaK) CSB-YP305578CMA
CSB-EP305578CMA
CSB-BP305578CMA
CSB-MP305578CMA
CSB-EP305578CMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Unknown protein CP 15 from 2D-PAGE CSB-YP305579CMA
CSB-EP305579CMA
CSB-BP305579CMA
CSB-MP305579CMA
CSB-EP305579CMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Unknown protein CP 41 from 2D-PAGE CSB-YP305580CMA
CSB-EP305580CMA
CSB-BP305580CMA
CSB-MP305580CMA
CSB-EP305580CMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 53 kDa major outer membrane protein CSB-YP305581PQP
CSB-EP305581PQP
CSB-BP305581PQP
CSB-MP305581PQP
CSB-EP305581PQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homarus americanus Cuticle protein AMP3 CSB-YP305582HWO
CSB-EP305582HWO
CSB-BP305582HWO
CSB-MP305582HWO
CSB-EP305582HWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antirrhinum majus Myb-related protein 308 (MYB308) CSB-YP305583DNE
CSB-EP305583DNE
CSB-BP305583DNE
CSB-MP305583DNE
CSB-EP305583DNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calloselasma rhodostoma Rhodocetin subunit alpha CSB-YP305584CBG
CSB-EP305584CBG
CSB-BP305584CBG
CSB-MP305584CBG
CSB-EP305584CBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein FAM189B (FAM189B), partial CSB-YP305585HU1
CSB-EP305585HU1
CSB-BP305585HU1
CSB-MP305585HU1
CSB-EP305585HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus furiosus DNA repair and recombination protein radB (radB) CSB-YP305586FHW
CSB-EP305586FHW
CSB-BP305586FHW
CSB-MP305586FHW
CSB-EP305586FHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 71 kDa F-actin-binding protein CSB-YP305587TLT
CSB-EP305587TLT
CSB-BP305587TLT
CSB-MP305587TLT
CSB-EP305587TLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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