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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0703.1 (MJ0703.1), partial CSB-YP305808MRU1
CSB-EP305808MRU1
CSB-BP305808MRU1
CSB-MP305808MRU1
CSB-EP305808MRU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ1282.1 (MJ1282.1), partial CSB-YP305809MRU1
CSB-EP305809MRU1
CSB-BP305809MRU1
CSB-MP305809MRU1
CSB-EP305809MRU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ1333.1 (MJ1333.1), partial CSB-YP305810MRU1
CSB-EP305810MRU1
CSB-BP305810MRU1
CSB-MP305810MRU1
CSB-EP305810MRU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Butyrate kinase (buk) CSB-YP305811CMA
CSB-EP305811CMA
CSB-BP305811CMA
CSB-MP305811CMA
CSB-EP305811CMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Unknown protein CP 10 from 2D-PAGE CSB-YP305812CMA
CSB-EP305812CMA
CSB-BP305812CMA
CSB-MP305812CMA
CSB-EP305812CMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Unknown protein CP 37 from 2D-PAGE CSB-YP305813CMA
CSB-EP305813CMA
CSB-BP305813CMA
CSB-MP305813CMA
CSB-EP305813CMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss Salmocidin-1 CSB-YP305814OEI
CSB-EP305814OEI
CSB-BP305814OEI
CSB-MP305814OEI
CSB-EP305814OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homarus americanus Cuticle protein AMP1A CSB-YP305815HWO
CSB-EP305815HWO
CSB-BP305815HWO
CSB-MP305815HWO
CSB-EP305815HWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alcaligenes xylosoxydans xylosoxydans Copper-containing nitrite reductase (nirK) CSB-YP305816AGR
CSB-EP305816AGR
CSB-BP305816AGR
CSB-MP305816AGR
CSB-EP305816AGR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canavalia cathartica Concanavalin-A CSB-YP305817CBZ
CSB-EP305817CBZ
CSB-BP305817CBZ
CSB-MP305817CBZ
CSB-EP305817CBZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Martes martes Double-headed protease inhibitor, submandibular gland CSB-YP305818MCH
CSB-EP305818MCH
CSB-BP305818MCH
CSB-MP305818MCH
CSB-EP305818MCH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phyllomedusa bicolor Dermaseptin-B6 CSB-YP305819PIP
CSB-EP305819PIP
CSB-BP305819PIP
CSB-MP305819PIP
CSB-EP305819PIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin B CSB-YP305820FPI
CSB-EP305820FPI
CSB-BP305820FPI
CSB-MP305820FPI
CSB-EP305820FPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium mucoroides Surface protein P34 (P34) CSB-YP305821DIA
CSB-EP305821DIA
CSB-BP305821DIA
CSB-MP305821DIA
CSB-EP305821DIA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Repressor-like protein SSo7c3 (SSO5984) CSB-YP305822FPM
CSB-EP305822FPM
CSB-BP305822FPM
CSB-MP305822FPM
CSB-EP305822FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Heat shock 22 kDa protein, mitochondrial CSB-YP305823SZY
CSB-EP305823SZY
CSB-BP305823SZY
CSB-MP305823SZY
CSB-EP305823SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dasyatis sabina Relaxin-like protein AGF CSB-YP305824DAM
CSB-EP305824DAM
CSB-BP305824DAM
CSB-MP305824DAM
CSB-EP305824DAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histone H3-5 (H3-5) CSB-YP305825SQX
CSB-EP305825SQX
CSB-BP305825SQX
CSB-MP305825SQX
CSB-EP305825SQX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syncephalastrum racemosum Nuclease CSB-YP305826FPW
CSB-EP305826FPW
CSB-BP305826FPW
CSB-MP305826FPW
CSB-EP305826FPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Actin-79 CSB-YP305827FIG
CSB-EP305827FIG
CSB-BP305827FIG
CSB-MP305827FIG
CSB-EP305827FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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