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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bothrops jararaca Thrombin-like enzyme TL-BJ 3 CSB-YP305788BUW
CSB-EP305788BUW
CSB-BP305788BUW
CSB-MP305788BUW
CSB-EP305788BUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Dihydrolipoamide-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex CSB-YP305789FIG
CSB-EP305789FIG
CSB-BP305789FIG
CSB-MP305789FIG
CSB-EP305789FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurus punctatus Histone H2B 1 CSB-YP305790ILX
CSB-EP305790ILX
CSB-BP305790ILX
CSB-MP305790ILX
CSB-EP305790ILX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Galleria mellonella Inducible serine protease inhibitor 2 CSB-YP305791GET
CSB-EP305791GET
CSB-BP305791GET
CSB-MP305791GET
CSB-EP305791GET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Putative odorant receptor 22c (Or22c), partial CSB-YP305792DLU1
CSB-EP305792DLU1
CSB-BP305792DLU1
CSB-MP305792DLU1
CSB-EP305792DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Odorant receptor 43b (Or43b), partial CSB-YP305793DLU1
CSB-EP305793DLU1
CSB-BP305793DLU1
CSB-MP305793DLU1
CSB-EP305793DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Putative odorant receptor 85e (Or85e), partial CSB-YP305794DLU1
CSB-EP305794DLU1
CSB-BP305794DLU1
CSB-MP305794DLU1
CSB-EP305794DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudopleuronectes americanus Pleurocidin (ple1) CSB-YP305795FGV
CSB-EP305795FGV
CSB-BP305795FGV
CSB-MP305795FGV
CSB-EP305795FGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant White spot syndrome virus 14.5 kDa structural polyprotein, partial CSB-YP305796WAM
CSB-EP305796WAM
CSB-BP305796WAM
CSB-MP305796WAM
CSB-EP305796WAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyles lineata Diuretic hormone 2 CSB-YP305797HRF
CSB-EP305797HRF
CSB-BP305797HRF
CSB-MP305797HRF
CSB-EP305797HRF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Boophilus microplus Protease inhibitor carrapatin CSB-YP305798BTV
CSB-EP305798BTV
CSB-BP305798BTV
CSB-MP305798BTV
CSB-EP305798BTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cerrophidion godmani Phospholipase A2 homolog, myotoxin II CSB-YP305799DRM
CSB-EP305799DRM
CSB-BP305799DRM
CSB-MP305799DRM
CSB-EP305799DRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Zinc metalloproteinase aureolysin (aur) CSB-YP305800FKZ
CSB-BP305800FKZ
CSB-MP305800FKZ
CSB-EP305800FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizophyllum commune Endoglucanase CSB-YP305801SXR
CSB-EP305801SXR
CSB-BP305801SXR
CSB-MP305801SXR
CSB-EP305801SXR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histone H3-6 (H3-6) CSB-YP305802SQX
CSB-EP305802SQX
CSB-BP305802SQX
CSB-MP305802SQX
CSB-EP305802SQX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ1249.1 (MJ1249.1), partial CSB-YP305803MRU1
CSB-EP305803MRU1
CSB-BP305803MRU1
CSB-MP305803MRU1
CSB-EP305803MRU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ1572.1 (MJ1572.1) CSB-YP305804MRU
CSB-EP305804MRU
CSB-BP305804MRU
CSB-MP305804MRU
CSB-EP305804MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mamestra brassicae Antennal odorant-binding protein CSB-YP305805MPT
CSB-EP305805MPT
CSB-BP305805MPT
CSB-MP305805MPT
CSB-EP305805MPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0323 (MJ0323), partial CSB-YP305806MRU1
CSB-EP305806MRU1
CSB-BP305806MRU1
CSB-MP305806MRU1
CSB-EP305806MRU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0332.1 (MJ0332.1), partial CSB-YP305807MRU1
CSB-EP305807MRU1
CSB-BP305807MRU1
CSB-MP305807MRU1
CSB-EP305807MRU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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