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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Orthochirus scrobiculosus Potassium channel toxin alpha-KTx 13.2 CSB-YP306051OFA
CSB-EP306051OFA
CSB-BP306051OFA
CSB-MP306051OFA
CSB-EP306051OFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oxyopes takobius M-oxotoxin-Ot2c CSB-YP306052OGA
CSB-EP306052OGA
CSB-BP306052OGA
CSB-MP306052OGA
CSB-EP306052OGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pireneitega luctuosa Delta-amaurobitoxin-Pl1a CSB-YP306053PKS
CSB-EP306053PKS
CSB-BP306053PKS
CSB-MP306053PKS
CSB-EP306053PKS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrolagus colliei Sperm protamine R3 isoform 1 CSB-YP306054HQY
CSB-EP306054HQY
CSB-BP306054HQY
CSB-MP306054HQY
CSB-EP306054HQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anarhichas minor Hemoglobin subunit beta-1 (hbb1) CSB-YP306055AJT
CSB-EP306055AJT
CSB-BP306055AJT
CSB-MP306055AJT
CSB-EP306055AJT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrobrachium rosenbergii FMRFamide-like neuropeptide FLP5 CSB-YP306056MAG
CSB-EP306056MAG
CSB-BP306056MAG
CSB-MP306056MAG
CSB-EP306056MAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amburana acreana Bowman-birk type proteinase inhibitor CSB-YP306057AIU
CSB-EP306057AIU
CSB-BP306057AIU
CSB-MP306057AIU
CSB-EP306057AIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobotrys dactyloides Superoxide dismutase [Mn/Fe] CSB-YP306058AOS
CSB-EP306058AOS
CSB-BP306058AOS
CSB-MP306058AOS
CSB-EP306058AOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Putative gustatory receptor 64a (Gr64a), partial CSB-YP306059DLU1
CSB-EP306059DLU1
CSB-BP306059DLU1
CSB-MP306059DLU1
CSB-EP306059DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lumbricus rubellus Fibrinolytic enzyme, isozyme C CSB-YP306060LRJ
CSB-EP306060LRJ
CSB-BP306060LRJ
CSB-MP306060LRJ
CSB-EP306060LRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma schmidti Mu-theraphotoxin-Hh2a CSB-YP306061HUL
CSB-EP306061HUL
CSB-BP306061HUL
CSB-MP306061HUL
CSB-EP306061HUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus acutifolius Bowman-Birk type proteinase inhibitor CSB-YP306062PGY
CSB-EP306062PGY
CSB-BP306062PGY
CSB-MP306062PGY
CSB-EP306062PGY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penaeus monodon FMRFamide-like neuropeptide FLP3 CSB-YP306063ETF
CSB-EP306063ETF
CSB-BP306063ETF
CSB-MP306063ETF
CSB-EP306063ETF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penaeus monodon FMRFamide-like neuropeptide FLP6 CSB-YP306064ETF
CSB-EP306064ETF
CSB-BP306064ETF
CSB-MP306064ETF
CSB-EP306064ETF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prunus persica Thaumatin-like protein 2 CSB-YP306065EZK
CSB-BP306065EZK
CSB-MP306065EZK
CSB-EP306065EZK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prunus persica Putative beta-D-xylosidase CSB-YP306066EZK
CSB-EP306066EZK
CSB-BP306066EZK
CSB-MP306066EZK
CSB-EP306066EZK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sarcophaga bullata FMRFamide-18 CSB-YP306067SCJ
CSB-EP306067SCJ
CSB-BP306067SCJ
CSB-MP306067SCJ
CSB-EP306067SCJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Limulus polyphemus Cuticle protein 16 isoform b CSB-YP306068LGC
CSB-EP306068LGC
CSB-BP306068LGC
CSB-MP306068LGC
CSB-EP306068LGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Limulus polyphemus Cuticle protein 7 isoform c CSB-YP306069LGC
CSB-EP306069LGC
CSB-BP306069LGC
CSB-MP306069LGC
CSB-EP306069LGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lupinus luteus Protein PR-L3 CSB-YP306070LRK
CSB-EP306070LRK
CSB-BP306070LRK
CSB-MP306070LRK
CSB-EP306070LRK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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