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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Brevibacillus brevis DNA protection during starvation protein (dps) CSB-YP306111BWK
CSB-EP306111BWK
CSB-BP306111BWK
CSB-MP306111BWK
CSB-EP306111BWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans UPF0312 protein CSB-YP306112DBE
CSB-EP306112DBE
CSB-BP306112DBE
CSB-MP306112DBE
CSB-EP306112DBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halobacterium sp. Halocin-C8 CSB-YP306113HBJ
CSB-EP306113HBJ
CSB-BP306113HBJ
CSB-MP306113HBJ
CSB-EP306113HBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naegleria fowleri Unknown protein NF041 from 2D-PAGE CSB-YP306114NAA
CSB-EP306114NAA
CSB-BP306114NAA
CSB-MP306114NAA
CSB-EP306114NAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heterodontus francisci IgW chain C region, secreted form 2 CSB-YP306115HGM
CSB-EP306115HGM
CSB-BP306115HGM
CSB-MP306115HGM
CSB-EP306115HGM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila teissieri Sex-regulated protein janus-A (janA) CSB-YP306116DMO
CSB-EP306116DMO
CSB-BP306116DMO
CSB-MP306116DMO
CSB-EP306116DMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prorocentrum triestinum Preblooming protein 2 CSB-YP306117PSK
CSB-EP306117PSK
CSB-BP306117PSK
CSB-MP306117PSK
CSB-EP306117PSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mastigocladus laminosus Cytochrome b6 (petB), partial CSB-YP306118MQJ1
CSB-EP306118MQJ1
CSB-BP306118MQJ1
CSB-MP306118MQJ1
CSB-EP306118MQJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mastigocladus laminosus Cytochrome b6-f complex subunit 7 (petM), partial CSB-YP306119MQJ
CSB-EP306119MQJ
CSB-BP306119MQJ
CSB-MP306119MQJ
CSB-EP306119MQJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinus comatus Antiviral protein CAP CSB-YP306120CPP
CSB-EP306120CPP
CSB-BP306120CPP
CSB-MP306120CPP
CSB-EP306120CPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mauritiana Ras-like protein 1 (Ras85D) CSB-YP306121DLT
CSB-EP306121DLT
CSB-BP306121DLT
CSB-MP306121DLT
CSB-EP306121DLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viola odorata Vodo peptide M CSB-YP306122VDB
CSB-EP306122VDB
CSB-BP306122VDB
CSB-MP306122VDB
CSB-EP306122VDB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Vam6-like protein CSB-YP306123MO
CSB-EP306123MO
CSB-BP306123MO
CSB-MP306123MO
CSB-EP306123MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gadus morhua Kininogen CSB-YP306124GER
CSB-EP306124GER
CSB-BP306124GER
CSB-MP306124GER
CSB-EP306124GER-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Subpeptin JM4-B CSB-YP306125BRI
CSB-EP306125BRI
CSB-BP306125BRI
CSB-MP306125BRI
CSB-EP306125BRI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Allium sativum Mannose-specific lectin (LECASAL) CSB-YP306126AHL
CSB-BP306126AHL
CSB-MP306126AHL
CSB-EP306126AHL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Implantin CSB-YP306127MO
CSB-EP306127MO
CSB-BP306127MO
CSB-MP306127MO
CSB-EP306127MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naegleria fowleri Unknown protein NF003 from 2D-PAGE CSB-YP306128NAA
CSB-EP306128NAA
CSB-BP306128NAA
CSB-MP306128NAA
CSB-EP306128NAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria reidyi Delta-ctenitoxin-Pr2d CSB-YP306129PHX
CSB-EP306129PHX
CSB-BP306129PHX
CSB-MP306129PHX
CSB-EP306129PHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces exfoliatus Leupeptin-inactivating enzyme 2 (lieB), partial CSB-YP306130SOC
CSB-EP306130SOC
CSB-BP306130SOC
CSB-MP306130SOC
CSB-EP306130SOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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