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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Protein rhsB (rhsB), partial CSB-YP325872ENV
CSB-EP325872ENV
CSB-BP325872ENV
CSB-MP325872ENV
CSB-EP325872ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Rod shape-determining protein mreC (mreC), partial CSB-YP325873ENV
CSB-EP325873ENV
CSB-BP325873ENV
CSB-MP325873ENV
CSB-EP325873ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage LP7 Protein GP2 (2) CSB-YP325874ECX
CSB-EP325874ECX
CSB-BP325874ECX
CSB-MP325874ECX
CSB-EP325874ECX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Curromycin resistance protein (cre) CSB-YP325876SOO
CSB-EP325876SOO
CSB-BP325876SOO
CSB-MP325876SOO
CSB-EP325876SOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Protein RecA (recA) CSB-YP325877BRJ
CSB-EP325877BRJ
CSB-BP325877BRJ
CSB-MP325877BRJ
CSB-EP325877BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nucleoprotein (N) CSB-YP325879BNN
CSB-EP325879BNN
CSB-BP325879BNN
CSB-MP325879BNN
CSB-EP325879BNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza C virus Hemagglutinin-esterase-fusion glycoprotein (HE), partial CSB-YP325880IKG
CSB-EP325880IKG
CSB-BP325880IKG
CSB-MP325880IKG
CSB-EP325880IKG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Protein CrtK (crtK), partial CSB-YP325881RLD1
CSB-EP325881RLD1
CSB-BP325881RLD1
CSB-MP325881RLD1
CSB-EP325881RLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 60S ribosomal protein L3-1 (ARP1) CSB-YP325883DOA
CSB-EP325883DOA
CSB-BP325883DOA
CSB-MP325883DOA
CSB-EP325883DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halobacterium salinarum Uncharacterized GTP-binding protein VNG_1111G (VNG_1111G) CSB-YP325884HTM
CSB-EP325884HTM
CSB-BP325884HTM
CSB-MP325884HTM
CSB-EP325884HTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Chorion protein S36 (Cp36) CSB-YP325885DMP
CSB-EP325885DMP
CSB-BP325885DMP
CSB-MP325885DMP
CSB-EP325885DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Sporulation-specific protein 16 (SPO16) CSB-YP325886SVG
CSB-EP325886SVG
CSB-BP325886SVG
CSB-MP325886SVG
CSB-EP325886SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus DNA primase UL70 (UL70), partial CSB-YP325887HWV
CSB-EP325887HWV
CSB-BP325887HWV
CSB-MP325887HWV
CSB-EP325887HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Maturase K (matK), partial CSB-YP325888HWQ
CSB-EP325888HWQ
CSB-BP325888HWQ
CSB-MP325888HWQ
CSB-EP325888HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Baseplate structural protein Gp51 (51) CSB-YP325889EDZ
CSB-EP325889EDZ
CSB-BP325889EDZ
CSB-MP325889EDZ
CSB-EP325889EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Armoracia rusticana Peroxidase C3 (PRXC3) CSB-YP325890AOC
CSB-EP325890AOC
CSB-BP325890AOC
CSB-MP325890AOC
CSB-EP325890AOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Duck hepatitis B virus Protein P (P), partial CSB-YP325891DHG
CSB-EP325891DHG
CSB-BP325891DHG
CSB-MP325891DHG
CSB-EP325891DHG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor RNA polymerase sigma factor whiG (whiG) CSB-YP325892FOB
CSB-EP325892FOB
CSB-BP325892FOB
CSB-MP325892FOB
CSB-EP325892FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vespa tropica Vespid chemotactic peptide T CSB-YP325893VBT
CSB-EP325893VBT
CSB-BP325893VBT
CSB-MP325893VBT
CSB-EP325893VBT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human parainfluenza 4a virus Nucleoprotein (N), partial CSB-YP325894HPK
CSB-EP325894HPK
CSB-BP325894HPK
CSB-MP325894HPK
CSB-EP325894HPK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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