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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Diaminopimelate decarboxylase (lysA) CSB-YP326079BRJ
CSB-EP326079BRJ
CSB-BP326079BRJ
CSB-MP326079BRJ
CSB-EP326079BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Proteasome component Y13 (PRE9) CSB-YP326080SVG
CSB-EP326080SVG
CSB-BP326080SVG
CSB-MP326080SVG
CSB-EP326080SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Neurogenic protein big brain (bib), partial CSB-YP326081DLU1
CSB-EP326081DLU1
CSB-BP326081DLU1
CSB-MP326081DLU1
CSB-EP326081DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribulose bisphosphate carboxylase small chain (rbcS) CSB-YP326082PXO
CSB-EP326082PXO
CSB-BP326082PXO
CSB-MP326082PXO
CSB-EP326082PXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Persea americana Endoglucanase 2 (CEL2) CSB-YP326083PGI
CSB-EP326083PGI
CSB-BP326083PGI
CSB-MP326083PGI
CSB-EP326083PGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana S-adenosylmethionine synthase 1 (SAM1) CSB-YP326084DOA
CSB-EP326084DOA
CSB-BP326084DOA
CSB-MP326084DOA
CSB-EP326084DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nodulation protein D 2 (nodD2) CSB-YP326085RKO
CSB-EP326085RKO
CSB-BP326085RKO
CSB-MP326085RKO
CSB-EP326085RKO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serine/threonine-protein phosphatase PP1 (5.9) CSB-YP326086TQV
CSB-EP326086TQV
CSB-BP326086TQV
CSB-MP326086TQV
CSB-EP326086TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Sucrase-isomaltase, intestinal (Si), partial CSB-YP326087RA
CSB-EP326087RA
CSB-BP326087RA
CSB-MP326087RA
CSB-EP326087RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Alginate biosynthesis transcriptional regulatory protein AlgB (algB) CSB-YP326088EZX
CSB-EP326088EZX
CSB-BP326088EZX
CSB-MP326088EZX
CSB-EP326088EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Galdieria sulphuraria Ribulose bisphosphate carboxylase small chain (rbcS) CSB-YP326089GAD
CSB-EP326089GAD
CSB-BP326089GAD
CSB-MP326089GAD
CSB-EP326089GAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Nitrogen metabolic regulation protein 1 (nmr-1) CSB-YP326090NHA
CSB-EP326090NHA
CSB-BP326090NHA
CSB-MP326090NHA
CSB-EP326090NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis GATA-binding factor 3 (gata3) CSB-YP326091XBE
CSB-EP326091XBE
CSB-BP326091XBE
CSB-MP326091XBE
CSB-EP326091XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica napus Serine/threonine-protein phosphatase PP1 CSB-YP326092BWD
CSB-EP326092BWD
CSB-BP326092BWD
CSB-MP326092BWD
CSB-EP326092BWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Autographa californica nuclear polyhedrosis virus Major immediate early protein (PE38) CSB-YP326095ARA
CSB-EP326095ARA
CSB-BP326095ARA
CSB-MP326095ARA
CSB-EP326095ARA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus 30S ribosomal protein S21 (rpsU) CSB-YP326096GFM
CSB-EP326096GFM
CSB-BP326096GFM
CSB-MP326096GFM
CSB-EP326096GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Periplasmic oligopeptide-binding protein (oppA) CSB-YP326098ENV
CSB-BP326098ENV
CSB-MP326098ENV
CSB-EP326098ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Peptidyl-prolyl cis-trans isomerase B (ppiB) CSB-YP326099ENV
CSB-EP326099ENV
CSB-BP326099ENV
CSB-MP326099ENV
CSB-EP326099ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ferric enterobactin transport ATP-binding protein FepC (fepC) CSB-YP326100ENV
CSB-EP326100ENV
CSB-BP326100ENV
CSB-MP326100ENV
CSB-EP326100ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polysialic acid transport ATP-binding protein KpsT (kpsT) CSB-YP326101ENL
CSB-EP326101ENL
CSB-BP326101ENL
CSB-MP326101ENL
CSB-EP326101ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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