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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YCL068C (YCL068C) CSB-YP326227SVG
CSB-EP326227SVG
CSB-BP326227SVG
CSB-MP326227SVG
CSB-EP326227SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YCL076W (YCL076W) CSB-YP326228SVG
CSB-EP326228SVG
CSB-BP326228SVG
CSB-MP326228SVG
CSB-EP326228SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YCR102C (YCR102C) CSB-YP326229SVG
CSB-EP326229SVG
CSB-BP326229SVG
CSB-MP326229SVG
CSB-EP326229SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YCR049C (YCR049C) CSB-YP326230SVG
CSB-EP326230SVG
CSB-BP326230SVG
CSB-MP326230SVG
CSB-EP326230SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized membrane protein YCR061W (YCR061W/YCR062W), partial CSB-YP326231SVG
CSB-EP326231SVG
CSB-BP326231SVG
CSB-MP326231SVG
CSB-EP326231SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YCR085W (YCR085W) CSB-YP326232SVG
CSB-EP326232SVG
CSB-BP326232SVG
CSB-MP326232SVG
CSB-EP326232SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cell division control protein 50 (CDC50), partial CSB-YP326233SVG1
CSB-EP326233SVG1
CSB-BP326233SVG1
CSB-MP326233SVG1
CSB-EP326233SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja naja Long neurotoxin 1 CSB-YP326234NAI
CSB-EP326234NAI
CSB-BP326234NAI
CSB-MP326234NAI
CSB-EP326234NAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dendroaspis polylepis polylepis Toxin MIT1 CSB-YP326235DBI
CSB-BP326235DBI
CSB-MP326235DBI
CSB-EP326235DBI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Claw keratin (CKER1) CSB-YP326236CH
CSB-EP326236CH
CSB-BP326236CH
CSB-MP326236CH
CSB-EP326236CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Phosphoribulokinase, chloroplastic (At1g32060) CSB-YP326237DOA
CSB-EP326237DOA
CSB-BP326237DOA
CSB-MP326237DOA
CSB-EP326237DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila pseudoobscura pseudoobscura Esterase-5B (Est-5B), partial CSB-YP326238DME
CSB-EP326238DME
CSB-BP326238DME
CSB-MP326238DME
CSB-EP326238DME-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UvrABC system protein A (uvrA) CSB-YP326239SYN
CSB-EP326239SYN
CSB-BP326239SYN
CSB-MP326239SYN
CSB-EP326239SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Lipopolysaccharide core heptosyltransferase rfaQ (rfaQ) CSB-YP326240ENV
CSB-EP326240ENV
CSB-BP326240ENV
CSB-MP326240ENV
CSB-EP326240ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli HTH-type transcriptional regulator galS (galS) CSB-YP326241ENV
CSB-EP326241ENV
CSB-BP326241ENV
CSB-MP326241ENV
CSB-EP326241ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Chitinase 12 (Cht12) CSB-YP326242OFG
CSB-EP326242OFG
CSB-BP326242OFG
CSB-MP326242OFG
CSB-EP326242OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Oryzain alpha chain (Os04g0650000, LOC_Os04g55650) CSB-YP326243OFG
CSB-EP326243OFG
CSB-BP326243OFG
CSB-MP326243OFG
CSB-EP326243OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gut-specific cysteine proteinase (cpr-1) CSB-YP326244CXY
CSB-EP326244CXY
CSB-BP326244CXY
CSB-MP326244CXY
CSB-EP326244CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP326245BRJ
CSB-EP326245BRJ
CSB-BP326245BRJ
CSB-MP326245BRJ
CSB-EP326245BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma mansoni Adenylate kinase CSB-YP326246SXQ
CSB-EP326246SXQ
CSB-BP326246SXQ
CSB-MP326246SXQ
CSB-EP326246SXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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