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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP326267IDS
CSB-EP326267IDS
CSB-BP326267IDS
CSB-MP326267IDS
CSB-EP326267IDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase basic protein 2 (PB2), partial CSB-YP326268IMI
CSB-EP326268IMI
CSB-BP326268IMI
CSB-MP326268IMI
CSB-EP326268IMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase basic protein 2 (PB2), partial CSB-YP326269IEP
CSB-EP326269IEP
CSB-BP326269IEP
CSB-MP326269IEP
CSB-EP326269IEP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus RNA-directed RNA polymerase catalytic subunit (PB1), partial CSB-YP326270IEZ
CSB-EP326270IEZ
CSB-BP326270IEZ
CSB-MP326270IEZ
CSB-EP326270IEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP326271IGP
CSB-EP326271IGP
CSB-BP326271IGP
CSB-MP326271IGP
CSB-EP326271IGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Non-structural protein 1 (NS) CSB-YP326272IBH
CSB-EP326272IBH
CSB-BP326272IBH
CSB-MP326272IBH
CSB-EP326272IBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Light-independent protochlorophyllide reductase subunit N (bchN) CSB-YP326274RLD
CSB-EP326274RLD
CSB-BP326274RLD
CSB-MP326274RLD
CSB-EP326274RLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Geranylgeranyl diphosphate reductase (bchP) CSB-YP326275RLD
CSB-EP326275RLD
CSB-BP326275RLD
CSB-MP326275RLD
CSB-EP326275RLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Chlorophyllide reductase 35.5 kDa chain (bchX) CSB-YP326276RLD
CSB-EP326276RLD
CSB-BP326276RLD
CSB-MP326276RLD
CSB-EP326276RLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein tonB (tonB), partial CSB-YP326277SYN
CSB-EP326277SYN
CSB-BP326277SYN
CSB-MP326277SYN
CSB-EP326277SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60 kDa chaperonin (groL), partial CSB-YP326278LDK
CSB-EP326278LDK
CSB-BP326278LDK
CSB-MP326278LDK
CSB-EP326278LDK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit 15 kDa protein A CSB-YP326279RB
CSB-EP326279RB
CSB-BP326279RB
CSB-MP326279RB
CSB-EP326279RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 6-phospho-beta-glucosidase (arbB) CSB-YP326280EGI
CSB-EP326280EGI
CSB-BP326280EGI
CSB-MP326280EGI
CSB-EP326280EGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Endopolygalacturonase II (pgaII) CSB-YP326281DOZ
CSB-EP326281DOZ
CSB-BP326281DOZ
CSB-MP326281DOZ
CSB-EP326281DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Endo-1,4-beta-xylanase B (xynB), partial CSB-YP326282BXZ
CSB-EP326282BXZ
CSB-BP326282BXZ
CSB-MP326282BXZ
CSB-EP326282BXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Magnesium-protoporphyrin O-methyltransferase (bchM) CSB-YP326283RLC
CSB-EP326283RLC
CSB-BP326283RLC
CSB-MP326283RLC
CSB-EP326283RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter agglomerans Nitrogenase iron protein (nifH) CSB-YP326284EKP
CSB-EP326284EKP
CSB-BP326284EKP
CSB-MP326284EKP
CSB-EP326284EKP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseobacter denitrificans Light-harvesting protein B-870 beta chain (pufB), partial CSB-YP326286RFJ
CSB-EP326286RFJ
CSB-BP326286RFJ
CSB-MP326286RFJ
CSB-EP326286RFJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus dysgalactiae subsp. equisimilis Deoxyribonuclease (sdc) CSB-YP326287SMK
CSB-EP326287SMK
CSB-BP326287SMK
CSB-MP326287SMK
CSB-EP326287SMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Periplaneta americana Hemolymph lipopolysaccharide-binding protein CSB-YP326288PFW
CSB-EP326288PFW
CSB-BP326288PFW
CSB-MP326288PFW
CSB-EP326288PFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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