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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mesocricetus auratus Fibroblast growth factor 1 (FGF1) CSB-YP327278MRG
CSB-EP327278MRG
CSB-BP327278MRG
CSB-MP327278MRG
CSB-EP327278MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variola virus 81 kDa A-type inclusion protein (A28L), partial CSB-YP327279VAR
CSB-EP327279VAR
CSB-BP327279VAR
CSB-MP327279VAR
CSB-EP327279VAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variola virus Protein M2 (M2L, O2L) CSB-YP327280VAR
CSB-EP327280VAR
CSB-BP327280VAR
CSB-MP327280VAR
CSB-EP327280VAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Type II secretion system protein L (pefL), partial CSB-YP327281XAY1
CSB-EP327281XAY1
CSB-BP327281XAY1
CSB-MP327281XAY1
CSB-EP327281XAY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Guanine nucleotide-binding protein alpha-7 subunit (gpaG) CSB-YP327282DKK
CSB-EP327282DKK
CSB-BP327282DKK
CSB-MP327282DKK
CSB-EP327282DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spodoptera exigua nuclear polyhedrosis virus Protein p10 (P10) CSB-YP327283SKH
CSB-EP327283SKH
CSB-BP327283SKH
CSB-MP327283SKH
CSB-EP327283SKH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Negative regulator of RAS-cAMP pathway (MKS1), partial CSB-YP327284SVG
CSB-EP327284SVG
CSB-BP327284SVG
CSB-MP327284SVG
CSB-EP327284SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Nucleoporin NIC96 (NIC96), partial CSB-YP327285SVG
CSB-EP327285SVG
CSB-BP327285SVG
CSB-MP327285SVG
CSB-EP327285SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit V (D)J recombination-activating protein 1, partial CSB-YP327286RB
CSB-EP327286RB
CSB-BP327286RB
CSB-MP327286RB
CSB-EP327286RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Phytochrome B (PHYB), partial CSB-YP327287FIG
CSB-EP327287FIG
CSB-BP327287FIG
CSB-MP327287FIG
CSB-EP327287FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Cyclin-dependent kinase 1 (cdk1) CSB-YP327288DKK
CSB-EP327288DKK
CSB-BP327288DKK
CSB-MP327288DKK
CSB-EP327288DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Coactosin (coaA) CSB-YP327289DKK
CSB-EP327289DKK
CSB-BP327289DKK
CSB-MP327289DKK
CSB-EP327289DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Tyrosine-protein phosphatase 2 (ptpB) CSB-YP327290DKK
CSB-EP327290DKK
CSB-BP327290DKK
CSB-MP327290DKK
CSB-EP327290DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Rho-related protein racD (racD) CSB-YP327291DKK
CSB-EP327291DKK
CSB-BP327291DKK
CSB-MP327291DKK
CSB-EP327291DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Nuclear cap-binding protein complex subunit 1 (STO1), partial CSB-YP327292SVG
CSB-EP327292SVG
CSB-BP327292SVG
CSB-MP327292SVG
CSB-EP327292SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lymantria dispar General odorant-binding protein CSB-YP327293LKU
CSB-EP327293LKU
CSB-BP327293LKU
CSB-MP327293LKU
CSB-EP327293LKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus atrox Zinc metalloproteinase/disintegrin CSB-YP327294DYC
CSB-EP327294DYC
CSB-BP327294DYC
CSB-MP327294DYC
CSB-EP327294DYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carassius auratus Deoxyribodipyrimidine photo-lyase (phr), partial CSB-YP327295DQG
CSB-EP327295DQG
CSB-BP327295DQG
CSB-MP327295DQG
CSB-EP327295DQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ADP-ribosylation factor-like protein 5 (arl-5) CSB-YP327296CXY
CSB-EP327296CXY
CSB-BP327296CXY
CSB-MP327296CXY
CSB-EP327296CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Deoxyribonuclease Tat-D (YBL055C) CSB-YP327297SVG
CSB-EP327297SVG
CSB-BP327297SVG
CSB-MP327297SVG
CSB-EP327297SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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