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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Glutathione exchanger 2 (GEX2), partial CSB-YP327501SVG
CSB-EP327501SVG
CSB-BP327501SVG
CSB-MP327501SVG
CSB-EP327501SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translation initiation factor IF-3, chloroplastic, partial CSB-YP327502EHU
CSB-EP327502EHU
CSB-BP327502EHU
CSB-MP327502EHU
CSB-EP327502EHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratitis capitata Ceratotoxin-A (CTXA1) CSB-YP327503DRF
CSB-EP327503DRF
CSB-BP327503DRF
CSB-MP327503DRF
CSB-EP327503DRF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 50S ribosomal protein L12-1, chloroplastic (RPL12A) CSB-YP327504DOA
CSB-EP327504DOA
CSB-BP327504DOA
CSB-MP327504DOA
CSB-EP327504DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus tremuloides Superoxide dismutase [Cu-Zn], chloroplastic (SODCP) CSB-YP327505EXR
CSB-EP327505EXR
CSB-BP327505EXR
CSB-MP327505EXR
CSB-EP327505EXR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paramecium bursaria Chlorella virus IL3A Modification methylase CviJI (CVIJIM) CSB-YP327506PCH
CSB-EP327506PCH
CSB-BP327506PCH
CSB-MP327506PCH
CSB-EP327506PCH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pathogenicity locus probable regulatory protein wtsA (wtsA) CSB-YP327507EGK
CSB-EP327507EGK
CSB-BP327507EGK
CSB-MP327507EGK
CSB-EP327507EGK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Nuclear migration protein JNM1 (JNM1) CSB-YP327508SVG
CSB-EP327508SVG
CSB-BP327508SVG
CSB-MP327508SVG
CSB-EP327508SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein rep (repA) CSB-YP327509BRE
CSB-EP327509BRE
CSB-BP327509BRE
CSB-MP327509BRE
CSB-EP327509BRE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycerate dehydrogenase CSB-YP327510HYC
CSB-EP327510HYC
CSB-BP327510HYC
CSB-MP327510HYC
CSB-EP327510HYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L11 (rplK) CSB-YP327511TNR
CSB-EP327511TNR
CSB-BP327511TNR
CSB-MP327511TNR
CSB-EP327511TNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium tRNA (guanine-N (1)-)-methyltransferase CSB-YP327512SXB
CSB-EP327512SXB
CSB-BP327512SXB
CSB-MP327512SXB
CSB-EP327512SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Liberibacter asiaticus 50S ribosomal protein L1 (rplA) CSB-YP327513LFN
CSB-EP327513LFN
CSB-BP327513LFN
CSB-MP327513LFN
CSB-EP327513LFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Preprotein translocase subunit SecE (secE), partial CSB-YP327514FLK
CSB-EP327514FLK
CSB-BP327514FLK
CSB-MP327514FLK
CSB-EP327514FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcription antitermination protein NusG (nusG) CSB-YP327515SUW
CSB-EP327515SUW
CSB-BP327515SUW
CSB-MP327515SUW
CSB-EP327515SUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium 50S ribosomal protein L10 (rplJ) CSB-YP327516MLN
CSB-EP327516MLN
CSB-BP327516MLN
CSB-MP327516MLN
CSB-EP327516MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P21 Head-tail preconnector protein GP5 (5) CSB-YP327517EDF
CSB-EP327517EDF
CSB-BP327517EDF
CSB-MP327517EDF
CSB-EP327517EDF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tomato leaf curl virus Replication-associated protein (C1) CSB-YP327518TGF
CSB-EP327518TGF
CSB-BP327518TGF
CSB-MP327518TGF
CSB-EP327518TGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant San Miguel sea lion virus serotype 1 Capsid protein (ORF2), partial CSB-YP327519SCC
CSB-EP327519SCC
CSB-BP327519SCC
CSB-MP327519SCC
CSB-EP327519SCC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant San Miguel sea lion virus serotype 4 Protein VP2 (ORF3) CSB-YP327520SCD
CSB-EP327520SCD
CSB-BP327520SCD
CSB-MP327520SCD
CSB-EP327520SCD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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