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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Phanerochaete chrysosporium Ligninase LG6 (GLG6) CSB-YP343487EUK
CSB-EP343487EUK
CSB-BP343487EUK
CSB-MP343487EUK
CSB-EP343487EUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tomato bushy stunt virus Movement protein p22 (ORF3) CSB-YP343488TGC
CSB-EP343488TGC
CSB-BP343488TGC
CSB-MP343488TGC
CSB-EP343488TGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 4 Ribonucleoside-diphosphate reductase small chain CSB-YP343489EFR
CSB-EP343489EFR
CSB-BP343489EFR
CSB-MP343489EFR
CSB-EP343489EFR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Alpha-defensin 15 (Defa15) CSB-YP343490MO
CSB-EP343490MO
CSB-BP343490MO
CSB-MP343490MO
CSB-EP343490MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyphantria cunea Hyphancin-3G CSB-YP343491HYB
CSB-EP343491HYB
CSB-BP343491HYB
CSB-MP343491HYB
CSB-EP343491HYB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ypbF (ypbF), partial CSB-YP343492BRJ1
CSB-EP343492BRJ1
CSB-BP343492BRJ1
CSB-MP343492BRJ1
CSB-EP343492BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Protease prsW (prsW), partial CSB-YP343493BRJ1
CSB-EP343493BRJ1
CSB-BP343493BRJ1
CSB-MP343493BRJ1
CSB-EP343493BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yphA (yphA), partial CSB-YP343494BRJ1
CSB-EP343494BRJ1
CSB-BP343494BRJ1
CSB-MP343494BRJ1
CSB-EP343494BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication protein E1 (E1), partial CSB-YP343495HMQ
CSB-EP343495HMQ
CSB-BP343495HMQ
CSB-MP343495HMQ
CSB-EP343495HMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication protein E1 (E1), partial CSB-YP343496HMU
CSB-EP343496HMU
CSB-BP343496HMU
CSB-MP343496HMU
CSB-EP343496HMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable protein E5 (E5) CSB-YP343497HOQ
CSB-EP343497HOQ
CSB-BP343497HOQ
CSB-MP343497HOQ
CSB-EP343497HOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major capsid protein L1 (L1), partial CSB-YP343498HMY
CSB-EP343498HMY
CSB-BP343498HMY
CSB-MP343498HMY
CSB-EP343498HMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Minor capsid protein L2 (L2), partial CSB-YP343499HMS
CSB-EP343499HMS
CSB-BP343499HMS
CSB-MP343499HMS
CSB-EP343499HMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major capsid protein L1 (L1) CSB-YP343500BKN
CSB-EP343500BKN
CSB-BP343500BKN
CSB-MP343500BKN
CSB-EP343500BKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major capsid protein L1 (L1), partial CSB-YP343501HNW
CSB-EP343501HNW
CSB-BP343501HNW
CSB-MP343501HNW
CSB-EP343501HNW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human papillomavirus type 61 Major capsid protein L1 (L1) CSB-YP343502HOH
CSB-EP343502HOH
CSB-BP343502HOH
CSB-MP343502HOH
CSB-EP343502HOH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Minor capsid protein L2 (L2), partial CSB-YP343503HNI
CSB-EP343503HNI
CSB-BP343503HNI
CSB-MP343503HNI
CSB-EP343503HNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yppE (yppE) CSB-YP343504BRJ
CSB-EP343504BRJ
CSB-BP343504BRJ
CSB-MP343504BRJ
CSB-EP343504BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yptA (yptA) CSB-YP343505BRJ
CSB-EP343505BRJ
CSB-BP343505BRJ
CSB-MP343505BRJ
CSB-EP343505BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 2-dehydro-3-deoxygluconokinase (kdgK) CSB-YP343506BRJ
CSB-EP343506BRJ
CSB-BP343506BRJ
CSB-MP343506BRJ
CSB-EP343506BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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