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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Putative transport permease ycf38 (ycf38), partial CSB-YP343343DZX1
CSB-EP343343DZX1
CSB-BP343343DZX1
CSB-MP343343DZX1
CSB-EP343343DZX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L22 (rplV) CSB-YP343344TNR
CSB-EP343344TNR
CSB-BP343344TNR
CSB-MP343344TNR
CSB-EP343344TNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus F serotype 40 Hexon-associated protein (PIX) CSB-YP343345HIR
CSB-EP343345HIR
CSB-BP343345HIR
CSB-MP343345HIR
CSB-EP343345HIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized 5.7 kDa protein in psbM-psbX intergenic region CSB-YP343346DZX
CSB-EP343346DZX
CSB-BP343346DZX
CSB-MP343346DZX
CSB-EP343346DZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Hydrogenase expression/formation protein hupG (hupG) CSB-YP343347BVW
CSB-EP343347BVW
CSB-BP343347BVW
CSB-MP343347BVW
CSB-EP343347BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 14-3-3-like protein GF14 epsilon (GRF10) CSB-YP343348DOA
CSB-EP343348DOA
CSB-BP343348DOA
CSB-MP343348DOA
CSB-EP343348DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein ycf21 (ycf21) CSB-YP343349DZX
CSB-EP343349DZX
CSB-BP343349DZX
CSB-MP343349DZX
CSB-EP343349DZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa DNA gyrase subunit A (gyrA), partial CSB-YP343350EZX
CSB-EP343350EZX
CSB-BP343350EZX
CSB-MP343350EZX
CSB-EP343350EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Camellia sinensis Chalcone synthase 3 (CHS3) CSB-YP343351CBP
CSB-EP343351CBP
CSB-BP343351CBP
CSB-MP343351CBP
CSB-EP343351CBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea nil Chalcone synthase A (CHSA) CSB-YP343352INC
CSB-EP343352INC
CSB-BP343352INC
CSB-MP343352INC
CSB-EP343352INC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea trifida Chalcone synthase B (CHSB) CSB-YP343353IKU
CSB-EP343353IKU
CSB-BP343353IKU
CSB-MP343353IKU
CSB-EP343353IKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Nonsense-mediated mRNA decay protein 3 (UPF3) CSB-YP343354SVG
CSB-EP343354SVG
CSB-BP343354SVG
CSB-MP343354SVG
CSB-EP343354SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica oleracea var. italica Glutathione S-transferase CSB-YP343355BPG
CSB-EP343355BPG
CSB-BP343355BPG
CSB-MP343355BPG
CSB-EP343355BPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Serine/threonine-protein phosphatase alpha-1 isoform (Pp1alpha-96A) CSB-YP343356DLU
CSB-EP343356DLU
CSB-BP343356DLU
CSB-MP343356DLU
CSB-EP343356DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetabularia cliftonii Serine/threonine-protein phosphatase PP1 isozyme 1 CSB-YP343357AAW
CSB-EP343357AAW
CSB-BP343357AAW
CSB-MP343357AAW
CSB-EP343357AAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Medicago sativa subsp. varia Serine/threonine-protein phosphatase PP1 (PP1) CSB-YP343358MNL
CSB-EP343358MNL
CSB-BP343358MNL
CSB-MP343358MNL
CSB-EP343358MNL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Triosephosphate isomerase, chloroplastic (TPIP1) CSB-YP343359FKI
CSB-EP343359FKI
CSB-BP343359FKI
CSB-MP343359FKI
CSB-EP343359FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Isoleucine--tRNA ligase, mitochondrial (ISM1), partial CSB-YP343360SVG
CSB-EP343360SVG
CSB-BP343360SVG
CSB-MP343360SVG
CSB-EP343360SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis 54S ribosomal protein L2, mitochondrial (MRPL2) CSB-YP343361KBK
CSB-EP343361KBK
CSB-BP343361KBK
CSB-MP343361KBK
CSB-EP343361KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YDL183C (YDL183C) CSB-YP343362SVG
CSB-EP343362SVG
CSB-BP343362SVG
CSB-MP343362SVG
CSB-EP343362SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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