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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae ATP-dependent permease PDR10 (PDR10), partial CSB-YP343547SVG
CSB-EP343547SVG
CSB-BP343547SVG
CSB-MP343547SVG
CSB-EP343547SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydra vulgaris Elongation factor 1-alpha CSB-YP343548HXN
CSB-EP343548HXN
CSB-BP343548HXN
CSB-MP343548HXN
CSB-EP343548HXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Endoprotease bli-4 (bli-4), partial CSB-YP343549CXY
CSB-EP343549CXY
CSB-BP343549CXY
CSB-MP343549CXY
CSB-EP343549CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Serine/threonine-protein kinase AFC3 (AFC3) CSB-YP343550DOA
CSB-EP343550DOA
CSB-BP343550DOA
CSB-MP343550DOA
CSB-EP343550DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GDP-mannose 6-dehydrogenase (algD) CSB-YP343551DPX
CSB-EP343551DPX
CSB-BP343551DPX
CSB-MP343551DPX
CSB-EP343551DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster E3 ubiquitin-protein ligase hyd (hyd), partial CSB-YP343552DLU
CSB-EP343552DLU
CSB-BP343552DLU
CSB-MP343552DLU
CSB-EP343552DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus phage HP1 Uncharacterized 7.9 kDa protein in int-C1 intergenic region CSB-YP343553HAN
CSB-EP343553HAN
CSB-BP343553HAN
CSB-MP343553HAN
CSB-EP343553HAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus phage HP1 Uncharacterized 19.2 kDa protein in cox-rep intergenic region CSB-YP343554HAN
CSB-EP343554HAN
CSB-BP343554HAN
CSB-MP343554HAN
CSB-EP343554HAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus phage HP1 Uncharacterized 14.9 kDa protein in rep-hol intergenic region CSB-YP343555HAN
CSB-EP343555HAN
CSB-BP343555HAN
CSB-MP343555HAN
CSB-EP343555HAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus phage HP1 Uncharacterized 19.2 kDa protein in rep-hol intergenic region CSB-YP343556HAN
CSB-EP343556HAN
CSB-BP343556HAN
CSB-MP343556HAN
CSB-EP343556HAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon kondoi 30S ribosomal protein S5 (rpsE) CSB-YP343559BMY
CSB-EP343559BMY
CSB-BP343559BMY
CSB-MP343559BMY
CSB-EP343559BMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Uteroferrin-associated basic protein 2 CSB-YP343560PI
CSB-EP343560PI
CSB-BP343560PI
CSB-MP343560PI
CSB-EP343560PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Valine--tRNA ligase (VALS) CSB-YP343561TPY
CSB-EP343561TPY
CSB-BP343561TPY
CSB-MP343561TPY
CSB-EP343561TPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease T (rnt) CSB-YP343562VFE
CSB-EP343562VFE
CSB-BP343562VFE
CSB-MP343562VFE
CSB-EP343562VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Shikimate dehydrogenase (aroE) CSB-YP343563BXE
CSB-EP343563BXE
CSB-BP343563BXE
CSB-MP343563BXE
CSB-EP343563BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Fructose-bisphosphate aldolase, cytoplasmic isozyme 2 CSB-YP343564EWE
CSB-EP343564EWE
CSB-BP343564EWE
CSB-MP343564EWE
CSB-EP343564EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Medicago sativa subsp. varia G2/mitotic-specific cyclin-2 CSB-YP343565MNL
CSB-EP343565MNL
CSB-BP343565MNL
CSB-MP343565MNL
CSB-EP343565MNL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Sedoheptulose-1,7-bisphosphatase, chloroplastic (At3g55800) CSB-YP343566DOA
CSB-EP343566DOA
CSB-BP343566DOA
CSB-MP343566DOA
CSB-EP343566DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glutamate--cysteine ligase, chloroplastic (GSH1) CSB-YP343567DOA
CSB-EP343567DOA
CSB-BP343567DOA
CSB-MP343567DOA
CSB-EP343567DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ABC transporter permease yvrN (yvrN), partial CSB-YP343568BRJ1
CSB-EP343568BRJ1
CSB-BP343568BRJ1
CSB-MP343568BRJ1
CSB-EP343568BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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