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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Geobacillus stearothermophilus PTS system mannitol-specific EIICB component (mtlA), partial CSB-YP343507GFM
CSB-EP343507GFM
CSB-BP343507GFM
CSB-MP343507GFM
CSB-EP343507GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP343508AXG
CSB-EP343508AXG
CSB-BP343508AXG
CSB-MP343508AXG
CSB-EP343508AXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trp operon leader peptide (trpL) CSB-YP343509DPS
CSB-EP343509DPS
CSB-BP343509DPS
CSB-MP343509DPS
CSB-EP343509DPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Platymonas subcordiformis Ribosomal protein S19, mitochondrial (RPS19) CSB-YP343510PMW
CSB-EP343510PMW
CSB-BP343510PMW
CSB-MP343510PMW
CSB-EP343510PMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Branchiostoma floridae Homeobox protein HOX3 CSB-YP343511BVY
CSB-EP343511BVY
CSB-BP343511BVY
CSB-MP343511BVY
CSB-EP343511BVY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division protein FtsZ (ftsZ) CSB-YP343512WAP
CSB-EP343512WAP
CSB-BP343512WAP
CSB-MP343512WAP
CSB-EP343512WAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Fructose-bisphosphate aldolase 2 (cbbA) CSB-YP343513RLC
CSB-EP343513RLC
CSB-BP343513RLC
CSB-MP343513RLC
CSB-EP343513RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Polyphosphatidylinositol phosphatase INP52 (INP52), partial CSB-YP343514SVG
CSB-EP343514SVG
CSB-BP343514SVG
CSB-MP343514SVG
CSB-EP343514SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transcriptional regulatory protein PHO23 (PHO23) CSB-YP343515SVG
CSB-EP343515SVG
CSB-BP343515SVG
CSB-MP343515SVG
CSB-EP343515SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Oligopeptide transport ATP-binding protein OppD (oppD) CSB-YP343516LBE
CSB-EP343516LBE
CSB-BP343516LBE
CSB-MP343516LBE
CSB-EP343516LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe AMP deaminase (ada1), partial CSB-YP343517SXV
CSB-EP343517SXV
CSB-BP343517SXV
CSB-MP343517SXV
CSB-EP343517SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 4-hydroxy-2-oxovalerate aldolase (nahM) CSB-YP343518FFZ
CSB-EP343518FFZ
CSB-BP343518FFZ
CSB-MP343518FFZ
CSB-EP343518FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Melatonin receptor type 1A-A (mtnr1aa), partial CSB-YP343519DIL1
CSB-EP343519DIL1
CSB-BP343519DIL1
CSB-MP343519DIL1
CSB-EP343519DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 2-oxoglutarate dehydrogenase E1 component (sucA), partial CSB-YP343520DXP
CSB-EP343520DXP
CSB-BP343520DXP
CSB-MP343520DXP
CSB-EP343520DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Phosphoenolpyruvate carboxylase, partial CSB-YP343521EWE
CSB-EP343521EWE
CSB-BP343521EWE
CSB-MP343521EWE
CSB-EP343521EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Medicago sativa Chalcone synthase 4-2 (CHS4-2) CSB-YP343522MQO
CSB-EP343522MQO
CSB-BP343522MQO
CSB-MP343522MQO
CSB-EP343522MQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vitis vinifera Leucoanthocyanidin dioxygenase CSB-YP343523VFQ
CSB-EP343523VFQ
CSB-BP343523VFQ
CSB-MP343523VFQ
CSB-EP343523VFQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Callistephus chinensis Dihydroflavonol-4-reductase (F) CSB-YP343524CAZ
CSB-EP343524CAZ
CSB-BP343524CAZ
CSB-MP343524CAZ
CSB-EP343524CAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Dihydroflavonol-4-reductase CSB-YP343525SZY
CSB-EP343525SZY
CSB-BP343525SZY
CSB-MP343525SZY
CSB-EP343525SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Medicago sativa Glycogen synthase kinase-3 homolog MsK-2 (MSK-2) CSB-YP343526MQO
CSB-EP343526MQO
CSB-BP343526MQO
CSB-MP343526MQO
CSB-EP343526MQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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