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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Coxiella burnetii Succinate dehydrogenase hydrophobic membrane anchor subunit (sdhD), partial CSB-YP344689DXP1
CSB-EP344689DXP1
CSB-BP344689DXP1
CSB-MP344689DXP1
CSB-EP344689DXP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fragaria ananassa Annexin-like protein RJ4 CSB-YP344690FEZ
CSB-EP344690FEZ
CSB-BP344690FEZ
CSB-MP344690FEZ
CSB-EP344690FEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Glycogen synthase kinase-3 (gskA) CSB-YP344691DKK
CSB-EP344691DKK
CSB-BP344691DKK
CSB-MP344691DKK
CSB-EP344691DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Light-independent protochlorophyllide reductase subunit N (chlN) CSB-YP344692EXV
CSB-EP344692EXV
CSB-BP344692EXV
CSB-MP344692EXV
CSB-EP344692EXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem I reaction center subunit IX (psaJ), partial CSB-YP344693EXV
CSB-EP344693EXV
CSB-BP344693EXV
CSB-MP344693EXV
CSB-EP344693EXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable RuBisCO transcriptional regulator (rbcR) CSB-YP344694EXV
CSB-EP344694EXV
CSB-BP344694EXV
CSB-MP344694EXV
CSB-EP344694EXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein ycf56 (ycf56) CSB-YP344695EXV
CSB-EP344695EXV
CSB-BP344695EXV
CSB-MP344695EXV
CSB-EP344695EXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease E homolog (rne), partial CSB-YP344696EXV
CSB-EP344696EXV
CSB-BP344696EXV
CSB-MP344696EXV
CSB-EP344696EXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP344697EXV1
CSB-EP344697EXV1
CSB-BP344697EXV1
CSB-MP344697EXV1
CSB-EP344697EXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribulose bisphosphate carboxylase small chain (rbcS) CSB-YP344698EXV
CSB-EP344698EXV
CSB-BP344698EXV
CSB-MP344698EXV
CSB-EP344698EXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S2, chloroplastic (rps2) CSB-YP344699EXV
CSB-EP344699EXV
CSB-BP344699EXV
CSB-MP344699EXV
CSB-EP344699EXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S20, chloroplastic (rps20) CSB-YP344700EXV
CSB-EP344700EXV
CSB-BP344700EXV
CSB-MP344700EXV
CSB-EP344700EXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Allophycocyanin alpha chain (apcA) CSB-YP344701EXV
CSB-EP344701EXV
CSB-BP344701EXV
CSB-MP344701EXV
CSB-EP344701EXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable protein-export membrane protein secG (secG), partial CSB-YP344702EXV1
CSB-EP344702EXV1
CSB-BP344702EXV1
CSB-MP344702EXV1
CSB-EP344702EXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem I reaction center subunit II (psaD) CSB-YP344703EXV
CSB-EP344703EXV
CSB-BP344703EXV
CSB-MP344703EXV
CSB-EP344703EXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S12, chloroplastic (rps12) CSB-YP344704EXV
CSB-EP344704EXV
CSB-BP344704EXV
CSB-MP344704EXV
CSB-EP344704EXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein translocase subunit SecY (secY), partial CSB-YP344705EXV
CSB-EP344705EXV
CSB-BP344705EXV
CSB-MP344705EXV
CSB-EP344705EXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S17, chloroplastic (rps17) CSB-YP344706EXV
CSB-EP344706EXV
CSB-BP344706EXV
CSB-MP344706EXV
CSB-EP344706EXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem II reaction center protein Z (psbZ), partial CSB-YP344707EXV1
CSB-EP344707EXV1
CSB-BP344707EXV1
CSB-MP344707EXV1
CSB-EP344707EXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem II reaction center protein H (psbH), partial CSB-YP344708EXV1
CSB-EP344708EXV1
CSB-BP344708EXV1
CSB-MP344708EXV1
CSB-EP344708EXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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