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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli dITP/XTP pyrophosphatase (rdgB) CSB-YP345967ENV
CSB-BP345967ENV
CSB-MP345967ENV
CSB-EP345967ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transcriptional regulatory protein qseB (qseB) CSB-YP345968ENV
CSB-EP345968ENV
CSB-BP345968ENV
CSB-MP345968ENV
CSB-EP345968ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Curli assembly protein CsgC (csgC) CSB-YP345969ENV
CSB-BP345969ENV
CSB-MP345969ENV
CSB-EP345969ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yfjJ (yfjJ) CSB-YP345970ENV
CSB-EP345970ENV
CSB-BP345970ENV
CSB-MP345970ENV
CSB-EP345970ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yfjL (yfjL) CSB-YP345971ENV
CSB-EP345971ENV
CSB-BP345971ENV
CSB-MP345971ENV
CSB-EP345971ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arsenical pump-driving ATPase (arsA) CSB-YP345972ENL
CSB-EP345972ENL
CSB-BP345972ENL
CSB-MP345972ENL
CSB-EP345972ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcription termination factor Rho (rho), partial CSB-YP345973FOI
CSB-EP345973FOI
CSB-BP345973FOI
CSB-MP345973FOI
CSB-EP345973FOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Box A-binding factor (srp), partial CSB-YP345974DLU
CSB-EP345974DLU
CSB-BP345974DLU
CSB-MP345974DLU
CSB-EP345974DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Heme exporter protein C (ccmC), partial CSB-YP345975PYC1
CSB-EP345975PYC1
CSB-BP345975PYC1
CSB-MP345975PYC1
CSB-EP345975PYC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guira guira Ovomucoid CSB-YP345976GDS
CSB-EP345976GDS
CSB-BP345976GDS
CSB-MP345976GDS
CSB-EP345976GDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bambusicola thoracicus Ovomucoid CSB-YP345977BSB
CSB-EP345977BSB
CSB-BP345977BSB
CSB-MP345977BSB
CSB-EP345977BSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium Probable ATP-dependent RNA helicase MG308 (MG308) CSB-YP345978MLN
CSB-EP345978MLN
CSB-BP345978MLN
CSB-MP345978MLN
CSB-EP345978MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Tubulin alpha chain CSB-YP345979BTT
CSB-EP345979BTT
CSB-BP345979BTT
CSB-MP345979BTT
CSB-EP345979BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. citri Proline iminopeptidase (pip) CSB-YP345980XAG
CSB-EP345980XAG
CSB-BP345980XAG
CSB-MP345980XAG
CSB-EP345980XAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Urease accessory protein UreF (ureF) CSB-YP345981YAH
CSB-EP345981YAH
CSB-BP345981YAH
CSB-MP345981YAH
CSB-EP345981YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA polymerase sigma factor rpoD (rpoD), partial CSB-YP345982FFT
CSB-EP345982FFT
CSB-BP345982FFT
CSB-MP345982FFT
CSB-EP345982FFT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. Nitrogenase iron protein (nifH) CSB-YP345983NEI
CSB-EP345983NEI
CSB-BP345983NEI
CSB-MP345983NEI
CSB-EP345983NEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 7 Tegument protein UL11 homolog (U71) CSB-YP345984HKB
CSB-EP345984HKB
CSB-BP345984HKB
CSB-MP345984HKB
CSB-EP345984HKB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine herpesvirus 1.1 Envelope glycoprotein M (gM), partial CSB-YP345985BJS
CSB-EP345985BJS
CSB-BP345985BJS
CSB-MP345985BJS
CSB-EP345985BJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine herpesvirus 1.1 Replication origin-binding protein (UL9), partial CSB-YP345986BJS
CSB-EP345986BJS
CSB-BP345986BJS
CSB-MP345986BJS
CSB-EP345986BJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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