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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Putative flavin carrier protein 3 (FLC3), partial CSB-YP346048SVG
CSB-EP346048SVG
CSB-BP346048SVG
CSB-MP346048SVG
CSB-EP346048SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Vacuolar fusion protein MON1 (MON1), partial CSB-YP346049SVG
CSB-EP346049SVG
CSB-BP346049SVG
CSB-MP346049SVG
CSB-EP346049SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YGL117W (YGL117W) CSB-YP346050SVG
CSB-EP346050SVG
CSB-BP346050SVG
CSB-MP346050SVG
CSB-EP346050SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Myosin light chain 1 (MLC1) CSB-YP346051SVG
CSB-EP346051SVG
CSB-BP346051SVG
CSB-MP346051SVG
CSB-EP346051SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ligase-interacting factor 1 (LIF1) CSB-YP346052SVG
CSB-EP346052SVG
CSB-BP346052SVG
CSB-MP346052SVG
CSB-EP346052SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YGL082W (YGL082W) CSB-YP346053SVG
CSB-EP346053SVG
CSB-BP346053SVG
CSB-MP346053SVG
CSB-EP346053SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Genetic interactor of prohibitin 7, mitochondrial (GEP7), partial CSB-YP346054SVG1
CSB-EP346054SVG1
CSB-BP346054SVG1
CSB-MP346054SVG1
CSB-EP346054SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae tRNA wybutosine-synthesizing protein 3 (TYW3) CSB-YP346055SVG
CSB-EP346055SVG
CSB-BP346055SVG
CSB-MP346055SVG
CSB-EP346055SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YGL041C (YGL041C), partial CSB-YP346056SVG1
CSB-EP346056SVG1
CSB-BP346056SVG1
CSB-MP346056SVG1
CSB-EP346056SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein BRP1 (BRP1) CSB-YP346057SVG
CSB-EP346057SVG
CSB-BP346057SVG
CSB-MP346057SVG
CSB-EP346057SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (ERG26) CSB-YP346058SVG
CSB-BP346058SVG
CSB-MP346058SVG
CSB-EP346058SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Nicotinamide-nucleotide adenylyltransferase 2 (NMA2) CSB-YP346059SVG
CSB-EP346059SVG
CSB-BP346059SVG
CSB-MP346059SVG
CSB-EP346059SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized membrane protein YGR026W (YGR026W), partial CSB-YP346060SVG1
CSB-EP346060SVG1
CSB-BP346060SVG1
CSB-MP346060SVG1
CSB-EP346060SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YGR035C (YGR035C) CSB-YP346061SVG
CSB-EP346061SVG
CSB-BP346061SVG
CSB-MP346061SVG
CSB-EP346061SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Bud site selection protein 9 (BUD9), partial CSB-YP346062SVG1
CSB-EP346062SVG1
CSB-BP346062SVG1
CSB-MP346062SVG1
CSB-EP346062SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Chromatin structure-remodeling complex subunit RSC1 (RSC1), partial CSB-YP346063SVG
CSB-EP346063SVG
CSB-BP346063SVG
CSB-MP346063SVG
CSB-EP346063SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Peroxisomal biogenesis factor 8 (PEX8), partial CSB-YP346064SVG
CSB-EP346064SVG
CSB-BP346064SVG
CSB-MP346064SVG
CSB-EP346064SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein NNF2 (NNF2), partial CSB-YP346065SVG
CSB-EP346065SVG
CSB-BP346065SVG
CSB-MP346065SVG
CSB-EP346065SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cardiolipin-specific deacylase 1, mitochondrial (CLD1) CSB-YP346066SVG
CSB-EP346066SVG
CSB-BP346066SVG
CSB-MP346066SVG
CSB-EP346066SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YGR117C (YGR117C) CSB-YP346067SVG
CSB-EP346067SVG
CSB-BP346067SVG
CSB-MP346067SVG
CSB-EP346067SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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