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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Phage-like element PBSX protein xkdN (xkdN) CSB-YP346534BRJ
CSB-EP346534BRJ
CSB-BP346534BRJ
CSB-MP346534BRJ
CSB-EP346534BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phage-like element PBSX protein xkdW (xkdW) CSB-YP346535BRJ
CSB-EP346535BRJ
CSB-BP346535BRJ
CSB-MP346535BRJ
CSB-EP346535BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Histone H2B.5 CSB-YP346536ZAX
CSB-EP346536ZAX
CSB-BP346536ZAX
CSB-MP346536ZAX
CSB-EP346536ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis Fragilysin (btfP) CSB-YP346537BDP
CSB-BP346537BDP
CSB-MP346537BDP
CSB-EP346537BDP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Ornithine decarboxylase antizyme (Oda) CSB-YP346538DLU
CSB-EP346538DLU
CSB-BP346538DLU
CSB-MP346538DLU
CSB-EP346538DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Shikimate dehydrogenase (aroD) CSB-YP346539BRJ
CSB-EP346539BRJ
CSB-BP346539BRJ
CSB-MP346539BRJ
CSB-EP346539BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP346540BRJ
CSB-EP346540BRJ
CSB-BP346540BRJ
CSB-MP346540BRJ
CSB-EP346540BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. NADP-specific glutamate dehydrogenase (gdhA) CSB-YP346541SSQ
CSB-EP346541SSQ
CSB-BP346541SSQ
CSB-MP346541SSQ
CSB-EP346541SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ypjA (ypjA), partial CSB-YP346542BRJ1
CSB-EP346542BRJ1
CSB-BP346542BRJ1
CSB-MP346542BRJ1
CSB-EP346542BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tubulin gamma chain CSB-YP346543EKM
CSB-EP346543EKM
CSB-BP346543EKM
CSB-MP346543EKM
CSB-EP346543EKM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus elongatus ATP-dependent Clp protease proteolytic subunit 1 (clpP1) CSB-YP346544FPY
CSB-EP346544FPY
CSB-BP346544FPY
CSB-MP346544FPY
CSB-EP346544FPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Cell wall-associated protease (wprA), partial CSB-YP346545BRJ
CSB-EP346545BRJ
CSB-BP346545BRJ
CSB-MP346545BRJ
CSB-EP346545BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0053 protein yrkA (yrkA), partial CSB-YP346546BRJ1
CSB-EP346546BRJ1
CSB-BP346546BRJ1
CSB-MP346546BRJ1
CSB-EP346546BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yqeB (yqeB), partial CSB-YP346547BRJ1
CSB-EP346547BRJ1
CSB-BP346547BRJ1
CSB-MP346547BRJ1
CSB-EP346547BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable RNA-binding protein YqeI (yqeI) CSB-YP346548BRJ
CSB-EP346548BRJ
CSB-BP346548BRJ
CSB-MP346548BRJ
CSB-EP346548BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative methyltransferase yqeM (yqeM) CSB-YP346549BRJ
CSB-EP346549BRJ
CSB-BP346549BRJ
CSB-MP346549BRJ
CSB-EP346549BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yqeW (yqeW), partial CSB-YP346550BRJ
CSB-EP346550BRJ
CSB-BP346550BRJ
CSB-MP346550BRJ
CSB-EP346550BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP346551BRJ
CSB-EP346551BRJ
CSB-BP346551BRJ
CSB-MP346551BRJ
CSB-EP346551BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative nucleotidase YqfW (yqfW) CSB-YP346552BRJ
CSB-EP346552BRJ
CSB-BP346552BRJ
CSB-MP346552BRJ
CSB-EP346552BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yqgL (yqgL) CSB-YP346553BRJ
CSB-EP346553BRJ
CSB-BP346553BRJ
CSB-MP346553BRJ
CSB-EP346553BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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