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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Setaria italica Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP349788SFQ
CSB-EP349788SFQ
CSB-BP349788SFQ
CSB-MP349788SFQ
CSB-EP349788SFQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Homeobox protein knotted-1-like 3 (KNOX3) CSB-YP349789ZAX
CSB-EP349789ZAX
CSB-BP349789ZAX
CSB-MP349789ZAX
CSB-EP349789ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Seed trypsin/chymotrypsin inhibitor IVB CSB-YP349790EWE
CSB-EP349790EWE
CSB-BP349790EWE
CSB-MP349790EWE
CSB-EP349790EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Jasus lalandii Crustacean hyperglycemic hormone CSB-YP349791JAI
CSB-EP349791JAI
CSB-BP349791JAI
CSB-MP349791JAI
CSB-EP349791JAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Astragalus bisulcatus Selenocysteine methyltransferase (SMTA) CSB-YP349792AQD
CSB-EP349792AQD
CSB-BP349792AQD
CSB-MP349792AQD
CSB-EP349792AQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus textile Omega-conotoxin TxVII CSB-YP349793DWJ
CSB-EP349793DWJ
CSB-BP349793DWJ
CSB-MP349793DWJ
CSB-EP349793DWJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga maritima Uncharacterized protein TM_0928 (TM_0928) CSB-YP349794TNJ
CSB-EP349794TNJ
CSB-BP349794TNJ
CSB-MP349794TNJ
CSB-EP349794TNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Avidin-related protein 7 (AVR7) CSB-YP349795CH
CSB-EP349795CH
CSB-BP349795CH
CSB-MP349795CH
CSB-EP349795CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Diaminobutyrate--2-oxoglutarate aminotransferase (dat) CSB-YP349796ABS
CSB-EP349796ABS
CSB-BP349796ABS
CSB-MP349796ABS
CSB-EP349796ABS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Photosystem I P700 chlorophyll a apoprotein A2 (psaB), partial CSB-YP349797DOA
CSB-EP349797DOA
CSB-BP349797DOA
CSB-MP349797DOA
CSB-EP349797DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP349798DOA
CSB-EP349798DOA
CSB-BP349798DOA
CSB-MP349798DOA
CSB-EP349798DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Photosystem II reaction center protein K (psbK) CSB-YP349799DOA
CSB-EP349799DOA
CSB-BP349799DOA
CSB-MP349799DOA
CSB-EP349799DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP349800DOA
CSB-EP349800DOA
CSB-BP349800DOA
CSB-MP349800DOA
CSB-EP349800DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 30S ribosomal protein S8, chloroplastic (rps8) CSB-YP349801DOA
CSB-EP349801DOA
CSB-BP349801DOA
CSB-MP349801DOA
CSB-EP349801DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Pyruvate synthase subunit porA (porA) CSB-YP349802MSR
CSB-EP349802MSR
CSB-BP349802MSR
CSB-MP349802MSR
CSB-EP349802MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sepia officinalis Hemocyanin, units E and F CSB-YP349803SYJ
CSB-EP349803SYJ
CSB-BP349803SYJ
CSB-MP349803SYJ
CSB-EP349803SYJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zymomonas mobilis Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP349804ZBM
CSB-EP349804ZBM
CSB-BP349804ZBM
CSB-MP349804ZBM
CSB-EP349804ZBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein TCL1B3 (Tcl1b3) CSB-YP349805MO
CSB-EP349805MO
CSB-BP349805MO
CSB-MP349805MO
CSB-EP349805MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Grammostola rosea Kappa-theraphotoxin-Gr1a CSB-YP349806GDE
CSB-EP349806GDE
CSB-BP349806GDE
CSB-MP349806GDE
CSB-EP349806GDE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deinococcus radiodurans Adenylyl-sulfate kinase (cysC) CSB-YP349807DJA
CSB-EP349807DJA
CSB-BP349807DJA
CSB-MP349807DJA
CSB-EP349807DJA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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