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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Listeria monocytogenes serovar 1/2a Probable cadmium-transporting ATPase (cadA), partial CSB-YP349748LPY1
CSB-EP349748LPY1
CSB-BP349748LPY1
CSB-MP349748LPY1
CSB-EP349748LPY1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sugar fermentation stimulation protein homolog (sfsA) CSB-YP349749EEH
CSB-EP349749EEH
CSB-BP349749EEH
CSB-MP349749EEH
CSB-EP349749EEH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viola odorata Cycloviolacin-O4 CSB-YP349750VDB
CSB-EP349750VDB
CSB-BP349750VDB
CSB-MP349750VDB
CSB-EP349750VDB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viola odorata Cycloviolacin-O8 (Voc1) CSB-YP349751VDB
CSB-EP349751VDB
CSB-BP349751VDB
CSB-MP349751VDB
CSB-EP349751VDB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hybanthus parviflorus Cyclotide hypa-A CSB-YP349752HQS
CSB-EP349752HQS
CSB-BP349752HQS
CSB-MP349752HQS
CSB-EP349752HQS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Proline--tRNA ligase (proS), partial CSB-YP349753HUV
CSB-EP349753HUV
CSB-BP349753HUV
CSB-MP349753HUV
CSB-EP349753HUV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Adenylosuccinate synthetase (purA) CSB-YP349754HUV
CSB-EP349754HUV
CSB-BP349754HUV
CSB-MP349754HUV
CSB-EP349754HUV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP349755HUV
CSB-EP349755HUV
CSB-BP349755HUV
CSB-MP349755HUV
CSB-EP349755HUV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative insulin-like peptide beta-type 6 (ins-5) CSB-YP349757CXY
CSB-EP349757CXY
CSB-BP349757CXY
CSB-MP349757CXY
CSB-EP349757CXY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thanatephorus cucumeris Laccase-1 (LCC1), partial CSB-YP349758TCE
CSB-EP349758TCE
CSB-BP349758TCE
CSB-MP349758TCE
CSB-EP349758TCE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Glycine--tRNA ligase (glyQS), partial CSB-YP349759TNT
CSB-EP349759TNT
CSB-BP349759TNT
CSB-MP349759TNT
CSB-EP349759TNT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Recombination protein RecR (recR) CSB-YP349760HUV
CSB-EP349760HUV
CSB-BP349760HUV
CSB-MP349760HUV
CSB-EP349760HUV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria splendida Caerin-2.1 CSB-YP349761LHS
CSB-EP349761LHS
CSB-BP349761LHS
CSB-MP349761LHS
CSB-EP349761LHS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria caerulea Caerin-4.2 CSB-YP349762LHF
CSB-EP349762LHF
CSB-BP349762LHF
CSB-MP349762LHF
CSB-EP349762LHF-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oldenlandia affinis Kalata-B1 (OAK1) CSB-YP349763OAZ
CSB-BP349763OAZ
CSB-MP349763OAZ
CSB-EP349763OAZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative carboxymethylenebutenolidase (ysgA) CSB-YP349764ENV
CSB-EP349764ENV
CSB-BP349764ENV
CSB-MP349764ENV
CSB-EP349764ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acid alpha-amylase CSB-YP349765DOZ
CSB-EP349765DOZ
CSB-BP349765DOZ
CSB-MP349765DOZ
CSB-EP349765DOZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein p13 MTCP-1 (MTCP1) CSB-YP349766HU
CSB-EP349766HU
CSB-BP349766HU
CSB-MP349766HU
CSB-EP349766HU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorella vulgaris ATP synthase subunit a, chloroplastic (atpI), partial CSB-YP349767DSP1
CSB-EP349767DSP1
CSB-BP349767DSP1
CSB-MP349767DSP1
CSB-EP349767DSP1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorella vulgaris Magnesium-chelatase subunit chlI (chlI) CSB-YP349768DSP
CSB-EP349768DSP
CSB-BP349768DSP
CSB-MP349768DSP
CSB-EP349768DSP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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