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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP350051BWZ
CSB-EP350051BWZ
CSB-BP350051BWZ
CSB-MP350051BWZ
CSB-EP350051BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum UPF0135 protein BU301 (BU301) CSB-YP350052BWZ
CSB-EP350052BWZ
CSB-BP350052BWZ
CSB-MP350052BWZ
CSB-EP350052BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Integration host factor subunit beta (ihfB) CSB-YP350053BWZ
CSB-EP350053BWZ
CSB-BP350053BWZ
CSB-MP350053BWZ
CSB-EP350053BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum High-affinity zinc uptake system membrane protein znuB (znuB), partial CSB-YP350054BWZ1
CSB-EP350054BWZ1
CSB-BP350054BWZ1
CSB-MP350054BWZ1
CSB-EP350054BWZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Cell division topological specificity factor (minE) CSB-YP350055BWZ
CSB-EP350055BWZ
CSB-BP350055BWZ
CSB-MP350055BWZ
CSB-EP350055BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Flagella basal body P-ring formation protein flgA (flgA) CSB-YP350056BWZ
CSB-EP350056BWZ
CSB-BP350056BWZ
CSB-MP350056BWZ
CSB-EP350056BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Flagellar P-ring protein (flgI) CSB-YP350057BWZ
CSB-EP350057BWZ
CSB-BP350057BWZ
CSB-MP350057BWZ
CSB-EP350057BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Porin-like protein BU359 (BU359) CSB-YP350058BWZ
CSB-EP350058BWZ
CSB-BP350058BWZ
CSB-MP350058BWZ
CSB-EP350058BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Cytosol aminopeptidase (pepA) CSB-YP350059BWZ
CSB-EP350059BWZ
CSB-BP350059BWZ
CSB-MP350059BWZ
CSB-EP350059BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP350060BWZ
CSB-EP350060BWZ
CSB-BP350060BWZ
CSB-MP350060BWZ
CSB-EP350060BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Ribosome maturation factor RimM (rimM) CSB-YP350061BWZ
CSB-EP350061BWZ
CSB-BP350061BWZ
CSB-MP350061BWZ
CSB-EP350061BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Alanine--tRNA ligase (alaS), partial CSB-YP350062BWZ
CSB-EP350062BWZ
CSB-BP350062BWZ
CSB-MP350062BWZ
CSB-EP350062BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Enolase (eno) CSB-YP350063BWZ
CSB-EP350063BWZ
CSB-BP350063BWZ
CSB-MP350063BWZ
CSB-EP350063BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Sulfate adenylyltransferase subunit 1 (cysN) CSB-YP350064BWZ
CSB-EP350064BWZ
CSB-BP350064BWZ
CSB-MP350064BWZ
CSB-EP350064BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum GTP-binding protein TypA/BipA homolog (typA), partial CSB-YP350065BWZ
CSB-EP350065BWZ
CSB-BP350065BWZ
CSB-MP350065BWZ
CSB-EP350065BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP350066BWZ
CSB-EP350066BWZ
CSB-BP350066BWZ
CSB-MP350066BWZ
CSB-EP350066BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum UPF0056 membrane protein BU449 (BU449), partial CSB-YP350067BWZ1
CSB-EP350067BWZ1
CSB-BP350067BWZ1
CSB-MP350067BWZ1
CSB-EP350067BWZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Membrane-bound lytic murein transglycosylase A homolog (mltA) CSB-YP350068BWZ
CSB-EP350068BWZ
CSB-BP350068BWZ
CSB-MP350068BWZ
CSB-EP350068BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Sulfurtransferase TusE (tusE) CSB-YP350069BWZ
CSB-EP350069BWZ
CSB-BP350069BWZ
CSB-MP350069BWZ
CSB-EP350069BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Uncharacterized protein BU473 (BU473) CSB-YP350070BWZ
CSB-EP350070BWZ
CSB-BP350070BWZ
CSB-MP350070BWZ
CSB-EP350070BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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