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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Holin-like protein CidA (cidA), partial CSB-YP350260SUL1
CSB-EP350260SUL1
CSB-BP350260SUL1
CSB-MP350260SUL1
CSB-EP350260SUL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP350261SWW
CSB-EP350261SWW
CSB-BP350261SWW
CSB-MP350261SWW
CSB-EP350261SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Na (+)/H (+) antiporter subunit A1, partial CSB-YP350262SKY
CSB-EP350262SKY
CSB-BP350262SKY
CSB-MP350262SKY
CSB-EP350262SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Na (+)/H (+) antiporter subunit C1, partial CSB-YP350263FKZ1
CSB-EP350263FKZ1
CSB-BP350263FKZ1
CSB-MP350263FKZ1
CSB-EP350263FKZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Na (+)/H (+) antiporter subunit E1, partial CSB-YP350264SKY1
CSB-EP350264SKY1
CSB-BP350264SKY1
CSB-MP350264SKY1
CSB-EP350264SKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Na (+)/H (+) antiporter subunit G1, partial CSB-YP350265SKY1
CSB-EP350265SKY1
CSB-BP350265SKY1
CSB-MP350265SKY1
CSB-EP350265SKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Probable DNA dC->dU-editing enzyme APOBEC3 (Apobec3) CSB-YP350266RA
CSB-EP350266RA
CSB-BP350266RA
CSB-MP350266RA
CSB-EP350266RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 50S ribosomal protein L4 (rplD) CSB-YP350267SXB
CSB-EP350267SXB
CSB-BP350267SXB
CSB-MP350267SXB
CSB-EP350267SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L4 (rplD) CSB-YP350268YAH
CSB-EP350268YAH
CSB-BP350268YAH
CSB-MP350268YAH
CSB-EP350268YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L24 (rplX) CSB-YP350269CQF
CSB-EP350269CQF
CSB-BP350269CQF
CSB-MP350269CQF
CSB-EP350269CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoplasma acidophilum 50S ribosomal protein L24P (rpl24p) CSB-YP350270TND
CSB-EP350270TND
CSB-BP350270TND
CSB-MP350270TND
CSB-EP350270TND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja philippinensis Short neurotoxin 1 CSB-YP350271NAN
CSB-EP350271NAN
CSB-BP350271NAN
CSB-MP350271NAN
CSB-EP350271NAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribonuclease P protein component 2 (rnp2) CSB-YP350272MSB
CSB-EP350272MSB
CSB-BP350272MSB
CSB-MP350272MSB
CSB-EP350272MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor 4 (lepA) CSB-YP350273EOD
CSB-EP350273EOD
CSB-BP350273EOD
CSB-MP350273EOD
CSB-EP350273EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Elongation factor 4 (lepA), partial CSB-YP350274TPP
CSB-EP350274TPP
CSB-BP350274TPP
CSB-MP350274TPP
CSB-EP350274TPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP350275SWW
CSB-EP350275SWW
CSB-BP350275SWW
CSB-MP350275SWW
CSB-EP350275SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Lipoate-protein ligase A (lplA) CSB-YP350276BFP
CSB-EP350276BFP
CSB-BP350276BFP
CSB-MP350276BFP
CSB-EP350276BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0304 protein yfbU (yfbU) CSB-YP350277SWW
CSB-EP350277SWW
CSB-BP350277SWW
CSB-MP350277SWW
CSB-EP350277SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium UPF0306 protein yhbP (yhbP) CSB-YP350278SXB
CSB-EP350278SXB
CSB-BP350278SXB
CSB-MP350278SXB
CSB-EP350278SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable disease resistance protein At1g12280 (At1g12280), partial CSB-YP350279DOA
CSB-EP350279DOA
CSB-BP350279DOA
CSB-MP350279DOA
CSB-EP350279DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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