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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methionine--tRNA ligase (metG), partial CSB-YP350177MVH
CSB-EP350177MVH
CSB-BP350177MVH
CSB-MP350177MVH
CSB-EP350177MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replicative DNA helicase (dnaB) CSB-YP350178MVH
CSB-EP350178MVH
CSB-BP350178MVH
CSB-MP350178MVH
CSB-EP350178MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Mb1354c (Mb1354c), partial CSB-YP350179MVH1
CSB-EP350179MVH1
CSB-BP350179MVH1
CSB-MP350179MVH1
CSB-EP350179MVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Endonuclease NucS (nucS) CSB-YP350180MVH
CSB-EP350180MVH
CSB-BP350180MVH
CSB-MP350180MVH
CSB-EP350180MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Mb3654 (Mb3654), partial CSB-YP350181MVH
CSB-EP350181MVH
CSB-BP350181MVH
CSB-MP350181MVH
CSB-EP350181MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Flagellar transcriptional regulator FlhD (flhD) CSB-YP350182PIJ
CSB-EP350182PIJ
CSB-BP350182PIJ
CSB-MP350182PIJ
CSB-EP350182PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Universal stress protein E (uspE) CSB-YP350183PIJ
CSB-EP350183PIJ
CSB-BP350183PIJ
CSB-MP350183PIJ
CSB-EP350183PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes D (2) dopamine receptor, partial CSB-YP350184EQV1
CSB-EP350184EQV1
CSB-BP350184EQV1
CSB-MP350184EQV1
CSB-EP350184EQV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Arginine/agmatine antiporter (adiC), partial CSB-YP350185ENV
CSB-EP350185ENV
CSB-BP350185ENV
CSB-MP350185ENV
CSB-EP350185ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorate reductase subunit alpha (clrA), partial CSB-YP350186IAF
CSB-EP350186IAF
CSB-BP350186IAF
CSB-MP350186IAF
CSB-EP350186IAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a UPF0291 protein lmo0496 (lmo0496) CSB-YP350187LPY
CSB-EP350187LPY
CSB-BP350187LPY
CSB-MP350187LPY
CSB-EP350187LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0291 protein MW1228 (MW1228) CSB-YP350188SUL
CSB-EP350188SUL
CSB-BP350188SUL
CSB-MP350188SUL
CSB-EP350188SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Arginase (arg) CSB-YP350189FLB
CSB-EP350189FLB
CSB-BP350189FLB
CSB-MP350189FLB
CSB-EP350189FLB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Ribosomal protein L11 methyltransferase (prmA) CSB-YP350190EYA
CSB-EP350190EYA
CSB-BP350190EYA
CSB-MP350190EYA
CSB-EP350190EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L11 (rplK) CSB-YP350191CQF
CSB-EP350191CQF
CSB-BP350191CQF
CSB-MP350191CQF
CSB-EP350191CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus TelA-like protein SAV1406 (SAV1406) CSB-YP350192SKX
CSB-EP350192SKX
CSB-BP350192SKX
CSB-MP350192SKX
CSB-EP350192SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum ATP synthase subunit 9, mitochondrial (ATP9), partial CSB-YP350193FIG1
CSB-EP350193FIG1
CSB-BP350193FIG1
CSB-MP350193FIG1
CSB-EP350193FIG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative pyridoxal phosphate-dependent acyltransferase (SA0508) CSB-YP350194SKY
CSB-EP350194SKY
CSB-BP350194SKY
CSB-MP350194SKY
CSB-EP350194SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Photosystem II reaction center protein L (psbL), partial CSB-YP350195DOA
CSB-EP350195DOA
CSB-BP350195DOA
CSB-MP350195DOA
CSB-EP350195DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem II reaction center protein L (psbL), partial CSB-YP350196LQQ
CSB-EP350196LQQ
CSB-BP350196LQQ
CSB-MP350196LQQ
CSB-EP350196LQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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