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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Zea mays Photosystem II reaction center protein L (psbL), partial CSB-YP350906ZAX
CSB-EP350906ZAX
CSB-BP350906ZAX
CSB-MP350906ZAX
CSB-EP350906ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Cytochrome b6 (petB), partial CSB-YP350907HWQ1
CSB-EP350907HWQ1
CSB-BP350907HWQ1
CSB-MP350907HWQ1
CSB-EP350907HWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sudan ebolavirus Pre-small/secreted glycoprotein (GP) CSB-YP350908SRD
CSB-EP350908SRD
CSB-BP350908SRD
CSB-MP350908SRD
CSB-EP350908SRD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative triosephosphate isomerase (tpiA) CSB-YP350909BAM
CSB-EP350909BAM
CSB-BP350909BAM
CSB-MP350909BAM
CSB-EP350909BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Adapter protein mecA (mecA) CSB-YP350910SUL
CSB-BP350910SUL
CSB-MP350910SUL
CSB-EP350910SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease BN (rbn) CSB-YP350911SWW
CSB-EP350911SWW
CSB-BP350911SWW
CSB-MP350911SWW
CSB-EP350911SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Androctonus mauretanicus mauretanicus Potassium channel toxin alpha-KTx 15.3 CSB-YP350912AKB
CSB-EP350912AKB
CSB-BP350912AKB
CSB-MP350912AKB
CSB-EP350912AKB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tityus obscurus Toxin To54 CSB-YP350913TET
CSB-EP350913TET
CSB-BP350913TET
CSB-MP350913TET
CSB-EP350913TET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Segregation and condensation protein A (scpA) CSB-YP350914SKX
CSB-EP350914SKX
CSB-BP350914SKX
CSB-MP350914SKX
CSB-EP350914SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dendroaspis angusticeps Muscarinic m1-toxin4 CSB-YP350915DBG
CSB-EP350915DBG
CSB-BP350915DBG
CSB-MP350915DBG
CSB-EP350915DBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Competence-stimulating peptide type 1 (comC1) CSB-YP350916FMW
CSB-EP350916FMW
CSB-BP350916FMW
CSB-MP350916FMW
CSB-EP350916FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buthus occitanus mardochei Toxin Boma6d CSB-YP350917BOB
CSB-EP350917BOB
CSB-BP350917BOB
CSB-MP350917BOB
CSB-EP350917BOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides limpidus limpidus Toxin II.9 CSB-YP350918CGA
CSB-EP350918CGA
CSB-BP350918CGA
CSB-MP350918CGA
CSB-EP350918CGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabuthus schlechteri Toxin PBITx1 CSB-YP350919PBU
CSB-EP350919PBU
CSB-BP350919PBU
CSB-MP350919PBU
CSB-EP350919PBU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tityus bahiensis Insect toxin TbIT-1 CSB-YP350920TEQ
CSB-EP350920TEQ
CSB-BP350920TEQ
CSB-MP350920TEQ
CSB-EP350920TEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP350921CQF
CSB-EP350921CQF
CSB-BP350921CQF
CSB-MP350921CQF
CSB-EP350921CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus furiosus DNA-directed RNA polymerase subunit N (rpoN) CSB-YP350923FHW
CSB-EP350923FHW
CSB-BP350923FHW
CSB-MP350923FHW
CSB-EP350923FHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ornithine aminotransferase 2 (rocD2) CSB-YP350924SKX
CSB-EP350924SKX
CSB-BP350924SKX
CSB-MP350924SKX
CSB-EP350924SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja kaouthia Cytotoxin 4 CSB-YP350925NAF
CSB-EP350925NAF
CSB-BP350925NAF
CSB-MP350925NAF
CSB-EP350925NAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja atra Cytotoxin SP15d CSB-YP350926NFG
CSB-EP350926NFG
CSB-BP350926NFG
CSB-MP350926NFG
CSB-EP350926NFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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