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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 50S ribosomal protein L4 (rplD) CSB-YP350967YAS
CSB-EP350967YAS
CSB-BP350967YAS
CSB-MP350967YAS
CSB-EP350967YAS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L24 (rplX) CSB-YP350968SMU
CSB-EP350968SMU
CSB-BP350968SMU
CSB-MP350968SMU
CSB-EP350968SMU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L24 (rplX) CSB-YP350969SJV
CSB-EP350969SJV
CSB-BP350969SJV
CSB-MP350969SJV
CSB-EP350969SJV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Lipid A export ATP-binding/permease protein MsbA (msbA), partial CSB-YP350970ENV1
CSB-EP350970ENV1
CSB-BP350970ENV1
CSB-MP350970ENV1
CSB-EP350970ENV1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja kaouthia Cobrotoxin CSB-YP350971NAF
CSB-EP350971NAF
CSB-BP350971NAF
CSB-MP350971NAF
CSB-EP350971NAF-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipoate-protein ligase A (lplA) CSB-YP350972EOD
CSB-EP350972EOD
CSB-BP350972EOD
CSB-MP350972EOD
CSB-EP350972EOD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP350973EOD1
CSB-EP350973EOD1
CSB-BP350973EOD1
CSB-MP350973EOD1
CSB-EP350973EOD1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor 4 (lepA), partial CSB-YP350974MLA
CSB-EP350974MLA
CSB-BP350974MLA
CSB-MP350974MLA
CSB-EP350974MLA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP350975SKY
CSB-EP350975SKY
CSB-BP350975SKY
CSB-MP350975SKY
CSB-EP350975SKY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP phosphoribosyltransferase (hisG) CSB-YP350976MLA
CSB-EP350976MLA
CSB-BP350976MLA
CSB-MP350976MLA
CSB-EP350976MLA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja atra Probable weak neurotoxin NNAM2I CSB-YP350977NFG
CSB-EP350977NFG
CSB-BP350977NFG
CSB-MP350977NFG
CSB-EP350977NFG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Selenide, water dikinase (selD) CSB-YP350978MRU
CSB-EP350978MRU
CSB-BP350978MRU
CSB-MP350978MRU
CSB-EP350978MRU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium UPF0307 protein YjgA (yjgA) CSB-YP350979SXB
CSB-EP350979SXB
CSB-BP350979SXB
CSB-MP350979SXB
CSB-EP350979SXB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus tokodaii Ribonuclease P protein component 1 (rnp1) CSB-YP350980FPN
CSB-EP350980FPN
CSB-BP350980FPN
CSB-MP350980FPN
CSB-EP350980FPN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA/RNA-binding protein Alba 1 (albA1) CSB-YP350981FPJ
CSB-EP350981FPJ
CSB-BP350981FPJ
CSB-MP350981FPJ
CSB-EP350981FPJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized protein SA0370 (SA0370) CSB-YP350982SKY
CSB-EP350982SKY
CSB-BP350982SKY
CSB-MP350982SKY
CSB-EP350982SKY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Histidinol dehydrogenase (hisD) CSB-YP350983MSB
CSB-EP350983MSB
CSB-BP350983MSB
CSB-MP350983MSB
CSB-EP350983MSB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative transport protein YbjL (ybjL), partial CSB-YP350984EGX
CSB-EP350984EGX
CSB-BP350984EGX
CSB-MP350984EGX
CSB-EP350984EGX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized protein SAV2627.1 (SAV2627.1) CSB-YP350985SKX
CSB-EP350985SKX
CSB-BP350985SKX
CSB-MP350985SKX
CSB-EP350985SKX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP350986RLU
CSB-EP350986RLU
CSB-BP350986RLU
CSB-MP350986RLU
CSB-EP350986RLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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