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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP351311SKY
CSB-EP351311SKY
CSB-BP351311SKY
CSB-MP351311SKY
CSB-EP351311SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Glycerol kinase (glpK), partial CSB-YP351312SMP
CSB-EP351312SMP
CSB-BP351312SMP
CSB-MP351312SMP
CSB-EP351312SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ethanolamine utilization protein EutS (eutS) CSB-YP351313ENV
CSB-EP351313ENV
CSB-BP351313ENV
CSB-MP351313ENV
CSB-EP351313ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv2242/MT2302 (Rv2242, MT2302) CSB-YP351314MVZ
CSB-EP351314MVZ
CSB-BP351314MVZ
CSB-MP351314MVZ
CSB-EP351314MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyl-transferase (pgsA2), partial CSB-YP351315MVH1
CSB-EP351315MVH1
CSB-BP351315MVH1
CSB-MP351315MVH1
CSB-EP351315MVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphatidate cytidylyltransferase (cdsA), partial CSB-YP351316MVZ
CSB-EP351316MVZ
CSB-BP351316MVZ
CSB-MP351316MVZ
CSB-EP351316MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphatidate cytidylyltransferase (cdsA), partial CSB-YP351317MVH
CSB-EP351317MVH
CSB-BP351317MVH
CSB-MP351317MVH
CSB-EP351317MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipid II flippase FtsW (ftsW), partial CSB-YP351318MVH
CSB-EP351318MVH
CSB-BP351318MVH
CSB-MP351318MVH
CSB-EP351318MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 60 kDa chaperonin (groL), partial CSB-YP351319SUL
CSB-EP351319SUL
CSB-BP351319SUL
CSB-MP351319SUL
CSB-EP351319SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 10 kDa chaperonin (groS) CSB-YP351320SMU
CSB-EP351320SMU
CSB-BP351320SMU
CSB-MP351320SMU
CSB-EP351320SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant CinA-like protein (cinA) CSB-YP351321MVH
CSB-EP351321MVH
CSB-BP351321MVH
CSB-MP351321MVH
CSB-EP351321MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease proteolytic subunit 2 (clpP2) CSB-YP351322MVH
CSB-EP351322MVH
CSB-BP351322MVH
CSB-MP351322MVH
CSB-EP351322MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP351323FMW
CSB-EP351323FMW
CSB-BP351323FMW
CSB-MP351323FMW
CSB-EP351323FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP351324SKY
CSB-EP351324SKY
CSB-BP351324SKY
CSB-MP351324SKY
CSB-EP351324SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP351325SMU
CSB-EP351325SMU
CSB-BP351325SMU
CSB-MP351325SMU
CSB-EP351325SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytidylate kinase (cmk) CSB-YP351326MVZ
CSB-EP351326MVZ
CSB-BP351326MVZ
CSB-MP351326MVZ
CSB-EP351326MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Cytidylate kinase (cmk) CSB-YP351327SUL
CSB-EP351327SUL
CSB-BP351327SUL
CSB-MP351327SUL
CSB-EP351327SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pantothenate kinase (coaA) CSB-YP351328MVH
CSB-EP351328MVH
CSB-BP351328MVH
CSB-MP351328MVH
CSB-EP351328MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Phosphopantetheine adenylyltransferase (coaD) CSB-YP351329BMO
CSB-EP351329BMO
CSB-BP351329BMO
CSB-MP351329BMO
CSB-EP351329BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphopantetheine adenylyltransferase (coaD) CSB-YP351330SKX
CSB-EP351330SKX
CSB-BP351330SKX
CSB-MP351330SKX
CSB-EP351330SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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