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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella suis biovar 1 3-dehydroquinate synthase (aroB) CSB-YP351290BMQ
CSB-EP351290BMQ
CSB-BP351290BMQ
CSB-MP351290BMQ
CSB-EP351290BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Arsenical pump membrane protein (arsB), partial CSB-YP351291SKX
CSB-EP351291SKX
CSB-BP351291SKX
CSB-MP351291SKX
CSB-EP351291SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate--ammonia ligase (asnA) CSB-YP351292EGX
CSB-EP351292EGX
CSB-BP351292EGX
CSB-MP351292EGX
CSB-EP351292EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate racemase (murI) CSB-YP351293SZB
CSB-EP351293SZB
CSB-BP351293SZB
CSB-MP351293SZB
CSB-EP351293SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glutamate racemase (murI) CSB-YP351294SKX
CSB-EP351294SKX
CSB-BP351294SKX
CSB-MP351294SKX
CSB-EP351294SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Glutamate racemase (murI) CSB-YP351295SMP
CSB-EP351295SMP
CSB-BP351295SMP
CSB-MP351295SMP
CSB-EP351295SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (scoB) CSB-YP351296MVZ
CSB-EP351296MVZ
CSB-BP351296MVZ
CSB-MP351296MVZ
CSB-EP351296MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase epsilon chain (atpC) CSB-YP351297MVZ
CSB-EP351297MVZ
CSB-BP351297MVZ
CSB-MP351297MVZ
CSB-EP351297MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase epsilon chain (atpC) CSB-YP351298SKY
CSB-EP351298SKY
CSB-BP351298SKY
CSB-MP351298SKY
CSB-EP351298SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae ATP synthase epsilon chain (atpC) CSB-YP351299SMP
CSB-EP351299SMP
CSB-BP351299SMP
CSB-MP351299SMP
CSB-EP351299SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase gamma chain (atpG) CSB-YP351300MVH
CSB-EP351300MVH
CSB-BP351300MVH
CSB-MP351300MVH
CSB-EP351300MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Potassium-transporting ATPase B chain 2 (kdpB2), partial CSB-YP351301SKY1
CSB-EP351301SKY1
CSB-BP351301SKY1
CSB-MP351301SKY1
CSB-EP351301SKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable cation-transporting ATPase F (ctpF), partial CSB-YP351302MVH
CSB-EP351302MVH
CSB-BP351302MVH
CSB-MP351302MVH
CSB-EP351302MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit c (atpE), partial CSB-YP351303MVH1
CSB-EP351303MVH1
CSB-BP351303MVH1
CSB-MP351303MVH1
CSB-EP351303MVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Mb1524 (Mb1524), partial CSB-YP351304MVH
CSB-EP351304MVH
CSB-BP351304MVH
CSB-MP351304MVH
CSB-EP351304MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacterioferritin (bfr) CSB-YP351305MVH
CSB-EP351305MVH
CSB-BP351305MVH
CSB-MP351305MVH
CSB-EP351305MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Uncharacterized protein NMA0420 (NMA0420), partial CSB-YP351306NGF1
CSB-EP351306NGF1
CSB-BP351306NGF1
CSB-MP351306NGF1
CSB-EP351306NGF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tagatose 1,6-diphosphate aldolase 2 (lacD2) CSB-YP351307SMU
CSB-EP351307SMU
CSB-BP351307SMU
CSB-MP351307SMU
CSB-EP351307SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carbamoyl-phosphate synthase small chain (carA) CSB-YP351309SMI
CSB-EP351309SMI
CSB-BP351309SMI
CSB-MP351309SMI
CSB-EP351309SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carbamoyl-phosphate synthase small chain (carA) CSB-YP351310SMT
CSB-EP351310SMT
CSB-BP351310SMT
CSB-MP351310SMT
CSB-EP351310SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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