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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP352933SZB
CSB-EP352933SZB
CSB-BP352933SZB
CSB-MP352933SZB
CSB-EP352933SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP352934EOD
CSB-EP352934EOD
CSB-BP352934EOD
CSB-MP352934EOD
CSB-EP352934EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon (cheB1) CSB-YP352935LEV
CSB-EP352935LEV
CSB-BP352935LEV
CSB-MP352935LEV
CSB-EP352935LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Chemotaxis response regulator protein-glutamate methylesterase of group 3 operon (cheB3) CSB-YP352936RLU
CSB-EP352936RLU
CSB-BP352936RLU
CSB-MP352936RLU
CSB-EP352936RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 50S ribosomal protein L32 (rpmF) CSB-YP352937TNS
CSB-EP352937TNS
CSB-BP352937TNS
CSB-MP352937TNS
CSB-EP352937TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 30S ribosomal protein S14 type Z (rpsZ) CSB-YP352938TNS
CSB-EP352938TNS
CSB-BP352938TNS
CSB-MP352938TNS
CSB-EP352938TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 30S ribosomal protein S6 (rpsF) CSB-YP352939TNS
CSB-EP352939TNS
CSB-BP352939TNS
CSB-MP352939TNS
CSB-EP352939TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein ApaG (apaG) CSB-YP352940SZB
CSB-EP352940SZB
CSB-BP352940SZB
CSB-MP352940SZB
CSB-EP352940SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chionodraco rastrospinosus Metallothionein B (mtb) CSB-YP352941CIN
CSB-EP352941CIN
CSB-BP352941CIN
CSB-MP352941CIN
CSB-EP352941CIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Endogenous retrovirus group K member 113 Gag polyprotein (HERVK_113) CSB-YP352942HU
CSB-BP352942HU
CSB-MP352942HU
CSB-EP352942HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus phage phivML3 Lysozyme (L3) CSB-YP352943LBL
CSB-EP352943LBL
CSB-BP352943LBL
CSB-MP352943LBL
CSB-EP352943LBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP352944EGX
CSB-EP352944EGX
CSB-BP352944EGX
CSB-MP352944EGX
CSB-EP352944EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Morone saxatilis Metallothionein B (mtb) CSB-YP352945MUL
CSB-EP352945MUL
CSB-BP352945MUL
CSB-MP352945MUL
CSB-EP352945MUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform (Ppp2cb) CSB-YP352946MO
CSB-EP352946MO
CSB-BP352946MO
CSB-MP352946MO
CSB-EP352946MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP352947TQN1
CSB-EP352947TQN1
CSB-BP352947TQN1
CSB-MP352947TQN1
CSB-EP352947TQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0324 inner membrane protein yeiH (yeiH), partial CSB-YP352948EOD
CSB-EP352948EOD
CSB-BP352948EOD
CSB-MP352948EOD
CSB-EP352948EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum 50S ribosomal protein L36, chloroplastic (rpl36) CSB-YP352949TQN
CSB-EP352949TQN
CSB-BP352949TQN
CSB-MP352949TQN
CSB-EP352949TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum 30S ribosomal protein S7, chloroplastic (rps7-A) CSB-YP352950TQN
CSB-EP352950TQN
CSB-BP352950TQN
CSB-MP352950TQN
CSB-EP352950TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Goat Inter-alpha-trypsin inhibitor CSB-YP352951GO
CSB-EP352951GO
CSB-BP352951GO
CSB-MP352951GO
CSB-EP352951GO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lama glama Tumor necrosis factor (TNF), partial CSB-YP352952LBS
CSB-EP352952LBS
CSB-BP352952LBS
CSB-MP352952LBS
CSB-EP352952LBS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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