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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP351737BQN
CSB-EP351737BQN
CSB-BP351737BQN
CSB-MP351737BQN
CSB-EP351737BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP351738TNS
CSB-EP351738TNS
CSB-BP351738TNS
CSB-MP351738TNS
CSB-EP351738TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris ATP phosphoribosyltransferase (hisG) CSB-YP351739DDH
CSB-EP351739DDH
CSB-BP351739DDH
CSB-MP351739DDH
CSB-EP351739DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ISPF) CSB-YP351740EWP
CSB-EP351740EWP
CSB-BP351740EWP
CSB-MP351740EWP
CSB-EP351740EWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Histidine--tRNA ligase (hisS) CSB-YP351741PIG
CSB-EP351741PIG
CSB-BP351741PIG
CSB-MP351741PIG
CSB-EP351741PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja naja Cytotoxin-like basic protein CSB-YP351742NAI
CSB-EP351742NAI
CSB-BP351742NAI
CSB-MP351742NAI
CSB-EP351742NAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptelytrops albolabris Disintegrin albolabrin CSB-YP351743DYI
CSB-EP351743DYI
CSB-BP351743DYI
CSB-MP351743DYI
CSB-EP351743DYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja annulifera Cytotoxin 11 CSB-YP351744NAC
CSB-EP351744NAC
CSB-BP351744NAC
CSB-MP351744NAC
CSB-EP351744NAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Secretion monitor (secM) CSB-YP351745ENV
CSB-EP351745ENV
CSB-BP351745ENV
CSB-MP351745ENV
CSB-EP351745ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Secretion monitor (secM) CSB-YP351746SZB
CSB-EP351746SZB
CSB-BP351746SZB
CSB-MP351746SZB
CSB-EP351746SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L5 (rplE) CSB-YP351747EGX
CSB-EP351747EGX
CSB-BP351747EGX
CSB-MP351747EGX
CSB-EP351747EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L5 (rplE) CSB-YP351748MVZ
CSB-EP351748MVZ
CSB-BP351748MVZ
CSB-MP351748MVZ
CSB-EP351748MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Phosphoglycerate kinase (pgk) CSB-YP351749BFP
CSB-EP351749BFP
CSB-BP351749BFP
CSB-MP351749BFP
CSB-EP351749BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Phosphoglycerate kinase (pgk) CSB-YP351750LAW
CSB-EP351750LAW
CSB-BP351750LAW
CSB-MP351750LAW
CSB-EP351750LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Phosphoglycerate kinase (pgk) CSB-YP351751TNS
CSB-EP351751TNS
CSB-BP351751TNS
CSB-MP351751TNS
CSB-EP351751TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 50S ribosomal protein L11 (rplK) CSB-YP351752OBN
CSB-EP351752OBN
CSB-BP351752OBN
CSB-MP351752OBN
CSB-EP351752OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 50S ribosomal protein L11 (rplK) CSB-YP351753TNS
CSB-EP351753TNS
CSB-BP351753TNS
CSB-MP351753TNS
CSB-EP351753TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP351754LEV
CSB-EP351754LEV
CSB-BP351754LEV
CSB-MP351754LEV
CSB-EP351754LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Histidinol dehydrogenase (hisD) CSB-YP351755BQN
CSB-EP351755BQN
CSB-BP351755BQN
CSB-MP351755BQN
CSB-EP351755BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Histidinol dehydrogenase (hisD) CSB-YP351756TNS
CSB-EP351756TNS
CSB-BP351756TNS
CSB-MP351756TNS
CSB-EP351756TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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