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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanothermobacter marburgensis Molybdenum-containing formylmethanofuran dehydrogenase 1 subunit C (fmdC) CSB-YP351696MSQ
CSB-EP351696MSQ
CSB-BP351696MSQ
CSB-MP351696MSQ
CSB-EP351696MSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens S-adenosylmethionine synthase (metK) CSB-YP351697GBK
CSB-EP351697GBK
CSB-BP351697GBK
CSB-MP351697GBK
CSB-EP351697GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O6:H1 Flavodoxin-1 (fldA) CSB-YP351698EGX
CSB-EP351698EGX
CSB-BP351698EGX
CSB-MP351698EGX
CSB-EP351698EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP351699GBK
CSB-EP351699GBK
CSB-BP351699GBK
CSB-MP351699GBK
CSB-EP351699GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus abyssi 30S ribosomal protein S4P (rps4p) CSB-YP351700FHV
CSB-EP351700FHV
CSB-BP351700FHV
CSB-MP351700FHV
CSB-EP351700FHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus furiosus Uncharacterized protein PF1875 (PF1875) CSB-YP351701FHW
CSB-EP351701FHW
CSB-BP351701FHW
CSB-MP351701FHW
CSB-EP351701FHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii 50S ribosomal protein L40e (rpl40e) CSB-YP351703FHX
CSB-EP351703FHX
CSB-BP351703FHX
CSB-MP351703FHX
CSB-EP351703FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii 30S ribosomal protein S11P (rps11p) CSB-YP351704FHX
CSB-EP351704FHX
CSB-BP351704FHX
CSB-MP351704FHX
CSB-EP351704FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus abyssi DNA-directed RNA polymerase subunit K (rpoK) CSB-YP351705FHV
CSB-EP351705FHV
CSB-BP351705FHV
CSB-MP351705FHV
CSB-EP351705FHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus tokodaii Membrane-associated ATPase C chain (atpP), partial CSB-YP351706FPN1
CSB-EP351706FPN1
CSB-BP351706FPN1
CSB-MP351706FPN1
CSB-EP351706FPN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L40e (rpl40e) CSB-YP351707MSB
CSB-EP351707MSB
CSB-BP351707MSB
CSB-MP351707MSB
CSB-EP351707MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Probable transcriptional regulatory protein DVU_2259 (DVU_2259) CSB-YP351708DDH
CSB-EP351708DDH
CSB-BP351708DDH
CSB-MP351708DDH
CSB-EP351708DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Probable transcriptional regulatory protein LIC_12886 (LIC_12886) CSB-YP351709LEV
CSB-EP351709LEV
CSB-BP351709LEV
CSB-MP351709LEV
CSB-EP351709LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable transcriptional regulatory protein WD_0484 (WD_0484) CSB-YP351710WAQ
CSB-EP351710WAQ
CSB-BP351710WAQ
CSB-MP351710WAQ
CSB-EP351710WAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus L-lactate dehydrogenase 3 (ldh3) CSB-YP351711BQN
CSB-EP351711BQN
CSB-BP351711BQN
CSB-MP351711BQN
CSB-EP351711BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii L-lactate dehydrogenase 1 (ldh1) CSB-YP351712LAW
CSB-EP351712LAW
CSB-BP351712LAW
CSB-MP351712LAW
CSB-EP351712LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate N-acetyltransferase (argJ) CSB-YP351713CQE
CSB-EP351713CQE
CSB-BP351713CQE
CSB-MP351713CQE
CSB-EP351713CQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yceQ (yceQ) CSB-YP351714EOD
CSB-EP351714EOD
CSB-BP351714EOD
CSB-MP351714EOD
CSB-EP351714EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Atropa belladonna Photosystem I iron-sulfur center (psaC) CSB-YP351715AQX
CSB-EP351715AQX
CSB-BP351715AQX
CSB-MP351715AQX
CSB-EP351715AQX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera elata subsp. hookeri Photosystem I iron-sulfur center (psaC) CSB-YP351716OAS
CSB-EP351716OAS
CSB-BP351716OAS
CSB-MP351716OAS
CSB-EP351716OAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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