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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Uncharacterized transporter Mb2022c (Mb2022c), partial CSB-YP351838MVH
CSB-EP351838MVH
CSB-BP351838MVH
CSB-MP351838MVH
CSB-EP351838MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionine import ATP-binding protein MetN (metN) CSB-YP351839EOD
CSB-EP351839EOD
CSB-BP351839EOD
CSB-MP351839EOD
CSB-EP351839EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable ribonucleotide transport ATP-binding protein mkl (mkl) CSB-YP351840MVH
CSB-EP351840MVH
CSB-BP351840MVH
CSB-MP351840MVH
CSB-EP351840MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Phosphate import ATP-binding protein PstB (pstB) CSB-YP351841BMQ
CSB-EP351841BMQ
CSB-BP351841BMQ
CSB-MP351841BMQ
CSB-EP351841BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a Phosphate import ATP-binding protein PstB 1 (pstB1) CSB-YP351842LGU
CSB-EP351842LGU
CSB-BP351842LGU
CSB-MP351842LGU
CSB-EP351842LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphate import ATP-binding protein PstB 1 (pstB1) CSB-YP351843SMI
CSB-EP351843SMI
CSB-BP351843SMI
CSB-MP351843SMI
CSB-EP351843SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphate import ATP-binding protein PstB 3 (pstB3) CSB-YP351844SMI
CSB-EP351844SMI
CSB-BP351844SMI
CSB-MP351844SMI
CSB-EP351844SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphate import ATP-binding protein PstB 1 (pstB1) CSB-YP351845SMT
CSB-EP351845SMT
CSB-BP351845SMT
CSB-MP351845SMT
CSB-EP351845SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UvrABC system protein A (uvrA), partial CSB-YP351846MVZ
CSB-EP351846MVZ
CSB-BP351846MVZ
CSB-MP351846MVZ
CSB-EP351846MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UvrABC system protein A (uvrA), partial CSB-YP351847FMW
CSB-EP351847FMW
CSB-BP351847FMW
CSB-MP351847FMW
CSB-EP351847FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable phospholipid import ATP-binding protein MlaF (mlaF) CSB-YP351848EGX
CSB-EP351848EGX
CSB-BP351848EGX
CSB-MP351848EGX
CSB-EP351848EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized ABC transporter ATP-binding protein YheS (yheS) CSB-YP351849EOD
CSB-EP351849EOD
CSB-BP351849EOD
CSB-MP351849EOD
CSB-EP351849EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Iron import ATP-binding/permease protein IrtB (irtB), partial CSB-YP351850MVH1
CSB-EP351850MVH1
CSB-BP351850MVH1
CSB-MP351850MVH1
CSB-EP351850MVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized ABC transporter ATP-binding protein Mb2353c (Mb2353c), partial CSB-YP351851MVH1
CSB-EP351851MVH1
CSB-BP351851MVH1
CSB-MP351851MVH1
CSB-EP351851MVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable propionyl-CoA carboxylase beta chain 6 (accD6) CSB-YP351852MVH
CSB-EP351852MVH
CSB-BP351852MVH
CSB-MP351852MVH
CSB-EP351852MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Acetate kinase (ackA) CSB-YP351853SXB
CSB-EP351853SXB
CSB-BP351853SXB
CSB-MP351853SXB
CSB-EP351853SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetate kinase (ackA) CSB-YP351854FMW
CSB-EP351854FMW
CSB-BP351854FMW
CSB-MP351854FMW
CSB-EP351854FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized N-acetyltransferase YhbS (yhbS) CSB-YP351855ENV
CSB-EP351855ENV
CSB-BP351855ENV
CSB-MP351855ENV
CSB-EP351855ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyltransferase YpeA (ypeA) CSB-YP351856EGX
CSB-EP351856EGX
CSB-BP351856EGX
CSB-MP351856EGX
CSB-EP351856EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyltransferase YpeA (ypeA) CSB-YP351857SWW
CSB-EP351857SWW
CSB-BP351857SWW
CSB-MP351857SWW
CSB-EP351857SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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