Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant 50S ribosomal protein L4 (rplD) CSB-YP352709MVZ
CSB-EP352709MVZ
CSB-BP352709MVZ
CSB-MP352709MVZ
CSB-EP352709MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L24 (rplX) CSB-YP352710SUL
CSB-EP352710SUL
CSB-BP352710SUL
CSB-MP352710SUL
CSB-EP352710SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 50S ribosomal protein L24 (rplX) CSB-YP352711RLU
CSB-EP352711RLU
CSB-BP352711RLU
CSB-MP352711RLU
CSB-EP352711RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipid A export ATP-binding/permease protein MsbA (msbA), partial CSB-YP352712EOD1
CSB-EP352712EOD1
CSB-BP352712EOD1
CSB-MP352712EOD1
CSB-EP352712EOD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP phosphoribosyltransferase (hisG) CSB-YP352713MVH
CSB-EP352713MVH
CSB-BP352713MVH
CSB-MP352713MVH
CSB-EP352713MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja atra Cobrotoxin CSB-YP352714NFG
CSB-BP352714NFG
CSB-MP352714NFG
CSB-EP352714NFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein tsgA (tsgA), partial CSB-YP352715EGX
CSB-EP352715EGX
CSB-BP352715EGX
CSB-MP352715EGX
CSB-EP352715EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor 4 (lepA) CSB-YP352716EGX
CSB-EP352716EGX
CSB-BP352716EGX
CSB-MP352716EGX
CSB-EP352716EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Elongation factor 4 (lepA), partial CSB-YP352717OBN
CSB-EP352717OBN
CSB-BP352717OBN
CSB-MP352717OBN
CSB-EP352717OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP352718SUL
CSB-EP352718SUL
CSB-BP352718SUL
CSB-MP352718SUL
CSB-EP352718SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens ATP phosphoribosyltransferase 1 (hisG1) CSB-YP352719GBK
CSB-EP352719GBK
CSB-BP352719GBK
CSB-MP352719GBK
CSB-EP352719GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Foldase protein prsA 2 (prsA2) CSB-YP352720SMU
CSB-EP352720SMU
CSB-BP352720SMU
CSB-MP352720SMU
CSB-EP352720SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP352721OBN1
CSB-EP352721OBN1
CSB-BP352721OBN1
CSB-MP352721OBN1
CSB-EP352721OBN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L4 (rplD) CSB-YP352722SMU
CSB-EP352722SMU
CSB-BP352722SMU
CSB-MP352722SMU
CSB-EP352722SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP352723GBK
CSB-EP352723GBK
CSB-BP352723GBK
CSB-MP352723GBK
CSB-EP352723GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Aquaporin Z (aqpZ), partial CSB-YP352724ENV1
CSB-EP352724ENV1
CSB-BP352724ENV1
CSB-MP352724ENV1
CSB-EP352724ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Histidinol dehydrogenase (hisD) CSB-YP352725GBK
CSB-EP352725GBK
CSB-BP352725GBK
CSB-MP352725GBK
CSB-EP352725GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative transport protein YidE (yidE), partial CSB-YP352726EOD
CSB-EP352726EOD
CSB-BP352726EOD
CSB-MP352726EOD
CSB-EP352726EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP352727MSB
CSB-EP352727MSB
CSB-BP352727MSB
CSB-MP352727MSB
CSB-EP352727MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidine--tRNA ligase (hisS) CSB-YP352728EOD
CSB-EP352728EOD
CSB-BP352728EOD
CSB-MP352728EOD
CSB-EP352728EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>