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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP352912WAQ
CSB-EP352912WAQ
CSB-BP352912WAQ
CSB-MP352912WAQ
CSB-EP352912WAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Nicotinamide riboside kinase (NRK1) CSB-YP352913DOT
CSB-EP352913DOT
CSB-BP352913DOT
CSB-MP352913DOT
CSB-EP352913DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glucans biosynthesis glucosyltransferase H (mdoH), partial CSB-YP352914ENV
CSB-EP352914ENV
CSB-BP352914ENV
CSB-MP352914ENV
CSB-EP352914ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant IlvGMEDA operon leader peptide (ilvL) CSB-YP352915EGX
CSB-EP352915EGX
CSB-BP352915EGX
CSB-MP352915EGX
CSB-EP352915EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant IlvGMEDA operon leader peptide (ilvL) CSB-YP352916SYN
CSB-EP352916SYN
CSB-BP352916SYN
CSB-MP352916SYN
CSB-EP352916SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative uncharacterized protein ORFC in retron EC67 CSB-YP352917ENL
CSB-EP352917ENL
CSB-BP352917ENL
CSB-MP352917ENL
CSB-EP352917ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication initiation protein (repE) CSB-YP352918SWW
CSB-EP352918SWW
CSB-BP352918SWW
CSB-MP352918SWW
CSB-EP352918SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Extatosoma tiaratum Hypertrehalosaemic neuropeptide CSB-YP352919EIW
CSB-EP352919EIW
CSB-BP352919EIW
CSB-MP352919EIW
CSB-EP352919EIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria chloris Caerin-1.7 CSB-YP352920LHG
CSB-EP352920LHG
CSB-BP352920LHG
CSB-MP352920LHG
CSB-EP352920LHG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Toxin CcdB (ccdB) CSB-YP352921EOD
CSB-EP352921EOD
CSB-BP352921EOD
CSB-MP352921EOD
CSB-EP352921EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M18 Exotoxin type A (speA) CSB-YP352922SMU
CSB-EP352922SMU
CSB-BP352922SMU
CSB-MP352922SMU
CSB-EP352922SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria splendida Caeridin-1.1/1.2/1.3 CSB-YP352923LHS
CSB-EP352923LHS
CSB-BP352923LHS
CSB-MP352923LHS
CSB-EP352923LHS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria splendida Caerin-1.1 CSB-YP352924LHS
CSB-EP352924LHS
CSB-BP352924LHS
CSB-MP352924LHS
CSB-EP352924LHS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria xanthomera Caeridin-1.4 CSB-YP352926LHT
CSB-EP352926LHT
CSB-BP352926LHT
CSB-MP352926LHT
CSB-EP352926LHT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capsule polysaccharide export inner-membrane protein kpsE (kpsE), partial CSB-YP352927EGX1
CSB-EP352927EGX1
CSB-BP352927EGX1
CSB-MP352927EGX1
CSB-EP352927EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Integrase/recombinase (int) CSB-YP352928EZW
CSB-EP352928EZW
CSB-BP352928EZW
CSB-MP352928EZW
CSB-EP352928EZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Two-component response regulator ARR12 (ARR12), partial CSB-YP352929DOA
CSB-EP352929DOA
CSB-BP352929DOA
CSB-MP352929DOA
CSB-EP352929DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sucrose operon repressor (cscR) CSB-YP352930ENL
CSB-EP352930ENL
CSB-BP352930ENL
CSB-MP352930ENL
CSB-EP352930ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant F7-2 fimbrial protein (F7-2) CSB-YP352931EGX
CSB-EP352931EGX
CSB-BP352931EGX
CSB-MP352931EGX
CSB-EP352931EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 30S ribosomal protein Thx (rpsU) CSB-YP352932TNS
CSB-EP352932TNS
CSB-BP352932TNS
CSB-MP352932TNS
CSB-EP352932TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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