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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Molybdenum cofactor biosynthesis protein A 2 (moaA2) CSB-YP354527MVH
CSB-EP354527MVH
CSB-BP354527MVH
CSB-MP354527MVH
CSB-EP354527MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP354528SUL
CSB-EP354528SUL
CSB-BP354528SUL
CSB-MP354528SUL
CSB-EP354528SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP354529SKY
CSB-EP354529SKY
CSB-BP354529SKY
CSB-MP354529SKY
CSB-EP354529SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable molybdenum cofactor guanylyltransferase (mobA) CSB-YP354530MVH
CSB-EP354530MVH
CSB-BP354530MVH
CSB-MP354530MVH
CSB-EP354530MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv2900c/MT2968 (Rv2900c, MT2968), partial CSB-YP354531MVZ
CSB-EP354531MVZ
CSB-BP354531MVZ
CSB-MP354531MVZ
CSB-EP354531MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Motility protein A (motA), partial CSB-YP354532HCQ1
CSB-EP354532HCQ1
CSB-BP354532HCQ1
CSB-MP354532HCQ1
CSB-EP354532HCQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative 3-methyladenine DNA glycosylase (SAV2344) CSB-YP354533SKX
CSB-EP354533SKX
CSB-BP354533SKX
CSB-MP354533SKX
CSB-EP354533SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Putative mannosyl-3-phosphoglycerate phosphatase (yedP) CSB-YP354534SXB
CSB-EP354534SXB
CSB-BP354534SXB
CSB-MP354534SXB
CSB-EP354534SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP354535MVZ
CSB-EP354535MVZ
CSB-BP354535MVZ
CSB-MP354535MVZ
CSB-EP354535MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein MraZ (mraZ) CSB-YP354536EOD
CSB-EP354536EOD
CSB-BP354536EOD
CSB-MP354536EOD
CSB-EP354536EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein mrp homolog (mrp) CSB-YP354537MVZ
CSB-EP354537MVZ
CSB-BP354537MVZ
CSB-MP354537MVZ
CSB-EP354537MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Peptide methionine sulfoxide reductase MsrA 2 (msrA2) CSB-YP354538SKY
CSB-EP354538SKY
CSB-BP354538SKY
CSB-MP354538SKY
CSB-EP354538SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP354539BMQ
CSB-EP354539BMQ
CSB-BP354539BMQ
CSB-MP354539BMQ
CSB-EP354539BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP354540SWW
CSB-EP354540SWW
CSB-BP354540SWW
CSB-MP354540SWW
CSB-EP354540SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP354541MVZ
CSB-EP354541MVZ
CSB-BP354541MVZ
CSB-MP354541MVZ
CSB-EP354541MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP354542FMW
CSB-EP354542FMW
CSB-BP354542FMW
CSB-MP354542FMW
CSB-EP354542FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP354543EGX
CSB-EP354543EGX
CSB-BP354543EGX
CSB-MP354543EGX
CSB-EP354543EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP354544SUL
CSB-EP354544SUL
CSB-BP354544SUL
CSB-MP354544SUL
CSB-EP354544SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE), partial CSB-YP354545MVZ
CSB-EP354545MVZ
CSB-BP354545MVZ
CSB-MP354545MVZ
CSB-EP354545MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable methylmalonyl-CoA mutase small subunit (mutA), partial CSB-YP354546MVH
CSB-EP354546MVH
CSB-BP354546MVH
CSB-MP354546MVH
CSB-EP354546MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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