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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pneumoniae DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP353613SMP
CSB-EP353613SMP
CSB-BP353613SMP
CSB-MP353613SMP
CSB-EP353613SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP353614XAY
CSB-EP353614XAY
CSB-BP353614XAY
CSB-MP353614XAY
CSB-EP353614XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP353615MVZ
CSB-EP353615MVZ
CSB-BP353615MVZ
CSB-MP353615MVZ
CSB-EP353615MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP353616SKX
CSB-EP353616SKX
CSB-BP353616SKX
CSB-MP353616SKX
CSB-EP353616SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP353617SMT
CSB-EP353617SMT
CSB-BP353617SMT
CSB-MP353617SMT
CSB-EP353617SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP353618SXB
CSB-EP353618SXB
CSB-BP353618SXB
CSB-MP353618SXB
CSB-EP353618SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP353619SXB
CSB-EP353619SXB
CSB-BP353619SXB
CSB-MP353619SXB
CSB-EP353619SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine synthase (metK) CSB-YP353620SWW
CSB-EP353620SWW
CSB-BP353620SWW
CSB-MP353620SWW
CSB-EP353620SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein spyM18_0604 (spyM18_0604) CSB-YP353621SMU
CSB-EP353621SMU
CSB-BP353621SMU
CSB-MP353621SMU
CSB-EP353621SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Mb1311c (Mb1311c), partial CSB-YP353622MVH1
CSB-EP353622MVH1
CSB-BP353622MVH1
CSB-MP353622MVH1
CSB-EP353622MVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable oxidoreductase ephD (ephD), partial CSB-YP353623MVH
CSB-EP353623MVH
CSB-BP353623MVH
CSB-MP353623MVH
CSB-EP353623MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized oxidoreductase Mb1385 (fabG2) CSB-YP353624MVH
CSB-EP353624MVH
CSB-BP353624MVH
CSB-MP353624MVH
CSB-EP353624MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized oxidoreductase Mb3820 (Mb3820) CSB-YP353625MVH
CSB-EP353625MVH
CSB-BP353625MVH
CSB-MP353625MVH
CSB-EP353625MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable protein-export membrane protein SecG (secG), partial CSB-YP353626MVH1
CSB-EP353626MVH1
CSB-BP353626MVH1
CSB-MP353626MVH1
CSB-EP353626MVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Response regulator uvrY (uvrY) CSB-YP353627EGX
CSB-EP353627EGX
CSB-BP353627EGX
CSB-MP353627EGX
CSB-EP353627EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable regulatory protein embR (embR) CSB-YP353628MVH
CSB-EP353628MVH
CSB-BP353628MVH
CSB-MP353628MVH
CSB-EP353628MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative hydrolase Rv0505c/MT0526 (Rv0505c, MT0526) CSB-YP353629MVZ
CSB-EP353629MVZ
CSB-BP353629MVZ
CSB-MP353629MVZ
CSB-EP353629MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DnaA initiator-associating protein DiaA (diaA) CSB-YP353630ENV
CSB-EP353630ENV
CSB-BP353630ENV
CSB-MP353630ENV
CSB-EP353630ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Anti-sigma-B factor antagonist (rsbV) CSB-YP353631SKX
CSB-EP353631SKX
CSB-BP353631SKX
CSB-MP353631SKX
CSB-EP353631SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Signal recognition particle receptor FtsY (ftsY) CSB-YP353633MVH
CSB-EP353633MVH
CSB-BP353633MVH
CSB-MP353633MVH
CSB-EP353633MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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