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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Neisseria meningitidis serogroup A / serotype 4A UPF0033 protein NMA0882 (NMA0882) CSB-YP353677NGF
CSB-EP353677NGF
CSB-BP353677NGF
CSB-MP353677NGF
CSB-EP353677NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0042 nucleotide-binding protein Rv1421/MT1464 (Rv1421, MT1464) CSB-YP353678MVZ
CSB-EP353678MVZ
CSB-BP353678MVZ
CSB-MP353678MVZ
CSB-EP353678MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0042 nucleotide-binding protein MW0727 (MW0727) CSB-YP353679SUL
CSB-EP353679SUL
CSB-BP353679SUL
CSB-MP353679SUL
CSB-EP353679SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant TVP38/TMEM64 family membrane protein Rv0625c/MT0653 (Rv0625c, MT0653), partial CSB-YP353680MVZ1
CSB-EP353680MVZ1
CSB-BP353680MVZ1
CSB-MP353680MVZ1
CSB-EP353680MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0045 protein Rv1898/MT1949 (Rv1898, MT1949) CSB-YP353681MVZ
CSB-EP353681MVZ
CSB-BP353681MVZ
CSB-MP353681MVZ
CSB-EP353681MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized sufE-like protein Rv3284/MT3383 (Rv3284, MT3383) CSB-YP353682MVZ
CSB-EP353682MVZ
CSB-BP353682MVZ
CSB-MP353682MVZ
CSB-EP353682MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0053 inner membrane protein ygdQ (ygdQ), partial CSB-YP353683ENV1
CSB-EP353683ENV1
CSB-BP353683ENV1
CSB-MP353683ENV1
CSB-EP353683ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Probable rRNA maturation factor (BMEI1974) CSB-YP353684BMO
CSB-EP353684BMO
CSB-BP353684BMO
CSB-MP353684BMO
CSB-EP353684BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable rRNA maturation factor (SAV1570) CSB-YP353685SKX
CSB-EP353685SKX
CSB-BP353685SKX
CSB-MP353685SKX
CSB-EP353685SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable rRNA maturation factor (SPy_0473, M5005_Spy0388) CSB-YP353686SMT
CSB-EP353686SMT
CSB-BP353686SMT
CSB-MP353686SMT
CSB-EP353686SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0056 inner membrane protein yhgN (yhgN), partial CSB-YP353687EOD1
CSB-EP353687EOD1
CSB-BP353687EOD1
CSB-MP353687EOD1
CSB-EP353687EOD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0060 membrane protein SAV2339 (SAV2339), partial CSB-YP353688SKX1
CSB-EP353688SKX1
CSB-BP353688SKX1
CSB-MP353688SKX1
CSB-EP353688SKX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA (Ile)-lysidine synthase CSB-YP353689MVH
CSB-EP353689MVH
CSB-BP353689MVH
CSB-MP353689MVH
CSB-EP353689MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0073 membrane protein Rv1085c/MT1117 (Rv1085c, MT1117), partial CSB-YP353690MVZ1
CSB-EP353690MVZ1
CSB-BP353690MVZ1
CSB-MP353690MVZ1
CSB-EP353690MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP353691SXB1
CSB-EP353691SXB1
CSB-BP353691SXB1
CSB-MP353691SXB1
CSB-EP353691SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP353692SUL1
CSB-EP353692SUL1
CSB-BP353692SUL1
CSB-MP353692SUL1
CSB-EP353692SUL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein SP_0007 (SP_0007) CSB-YP353693FMW
CSB-EP353693FMW
CSB-BP353693FMW
CSB-MP353693FMW
CSB-EP353693FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein htpG (htpG), partial CSB-YP353694MVZ
CSB-EP353694MVZ
CSB-BP353694MVZ
CSB-MP353694MVZ
CSB-EP353694MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nickel-responsive regulator (nikR) CSB-YP353695SWW
CSB-EP353695SWW
CSB-BP353695SWW
CSB-MP353695SWW
CSB-EP353695SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Probable hydrogenase nickel incorporation protein HybF (hybF) CSB-YP353696SXB
CSB-EP353696SXB
CSB-BP353696SXB
CSB-MP353696SXB
CSB-EP353696SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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