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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant NAD-dependent protein deacylase (cobB) CSB-YP354221MVH
CSB-EP354221MVH
CSB-BP354221MVH
CSB-MP354221MVH
CSB-EP354221MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DnaA initiator-associating protein DiaA (diaA) CSB-YP354222EOD
CSB-EP354222EOD
CSB-BP354222EOD
CSB-MP354222EOD
CSB-EP354222EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Protein smg homolog (smg) CSB-YP354223NGG
CSB-EP354223NGG
CSB-BP354223NGG
CSB-MP354223NGG
CSB-EP354223NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Superoxide dismutase [Mn/Fe] 2 (sodM) CSB-YP354224SKX
CSB-EP354224SKX
CSB-BP354224SKX
CSB-MP354224SKX
CSB-EP354224SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Anti-sigma-B factor antagonist (rsbV) CSB-YP354225SKY
CSB-EP354225SKY
CSB-BP354225SKY
CSB-MP354225SKY
CSB-EP354225SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Signal recognition particle protein (ffh), partial CSB-YP354226MVZ
CSB-EP354226MVZ
CSB-BP354226MVZ
CSB-MP354226MVZ
CSB-EP354226MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Single-stranded DNA-binding protein 1 (ssb1) CSB-BP354227SMH
CSB-MP354227SMH
CSB-EP354227SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 SsrA-binding protein (smpB) CSB-YP354228BMQ
CSB-EP354228BMQ
CSB-BP354228BMQ
CSB-MP354228BMQ
CSB-EP354228BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus SsrA-binding protein (smpB) CSB-YP354229SKX
CSB-EP354229SKX
CSB-BP354229SKX
CSB-MP354229SKX
CSB-EP354229SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant SsrA-binding protein (smpB) CSB-YP354230SMI
CSB-EP354230SMI
CSB-BP354230SMI
CSB-MP354230SMI
CSB-EP354230SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv1339/MT1380 (Rv1339, MT1380) CSB-YP354231MVZ
CSB-EP354231MVZ
CSB-BP354231MVZ
CSB-MP354231MVZ
CSB-EP354231MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cystathionine gamma-synthase (metB) CSB-YP354232MVH
CSB-EP354232MVH
CSB-BP354232MVH
CSB-MP354232MVH
CSB-EP354232MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized SURF1-like protein Mb2259 (Mb2259), partial CSB-YP354234MVH1
CSB-EP354234MVH1
CSB-BP354234MVH1
CSB-MP354234MVH1
CSB-EP354234MVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Sec-independent protein translocase protein TatA (tatA), partial CSB-YP354235BMO
CSB-EP354235BMO
CSB-BP354235BMO
CSB-MP354235BMO
CSB-EP354235BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Sec-independent protein translocase protein TatA (tatA), partial CSB-YP354236NGG
CSB-EP354236NGG
CSB-BP354236NGG
CSB-MP354236NGG
CSB-EP354236NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-threonine dehydratase biosynthetic IlvA (ilvA) CSB-YP354237MVH
CSB-EP354237MVH
CSB-BP354237MVH
CSB-MP354237MVH
CSB-EP354237MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Threonine synthase (thrC) CSB-YP354238MVH
CSB-EP354238MVH
CSB-BP354238MVH
CSB-MP354238MVH
CSB-EP354238MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (thiD) CSB-YP354239MVH
CSB-EP354239MVH
CSB-BP354239MVH
CSB-MP354239MVH
CSB-EP354239MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Thiamine-phosphate synthase (thiE) CSB-YP354240SKY
CSB-EP354240SKY
CSB-BP354240SKY
CSB-MP354240SKY
CSB-EP354240SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Hydroxyethylthiazole kinase (thiM) CSB-YP354241SKX
CSB-EP354241SKX
CSB-BP354241SKX
CSB-MP354241SKX
CSB-EP354241SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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