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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli UPF0056 inner membrane protein yhgN (yhgN), partial CSB-YP354285ENV1
CSB-EP354285ENV1
CSB-BP354285ENV1
CSB-MP354285ENV1
CSB-EP354285ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0060 membrane protein Mb2672c (Mb2672c), partial CSB-YP354286MVH1
CSB-EP354286MVH1
CSB-BP354286MVH1
CSB-MP354286MVH1
CSB-EP354286MVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA (Ile)-lysidine synthase CSB-YP354287MVZ
CSB-EP354287MVZ
CSB-BP354287MVZ
CSB-MP354287MVZ
CSB-EP354287MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0073 inner membrane protein yqfA (yqfA), partial CSB-YP354288EOD1
CSB-EP354288EOD1
CSB-BP354288EOD1
CSB-MP354288EOD1
CSB-EP354288EOD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative HTH-type transcriptional regulator Rv1287/MT1325 (Rv1287, MT1325) CSB-YP354289MVZ
CSB-EP354289MVZ
CSB-BP354289MVZ
CSB-MP354289MVZ
CSB-EP354289MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative HTH-type transcriptional regulator Mb1318 (Mb1318) CSB-YP354290MVH
CSB-EP354290MVH
CSB-BP354290MVH
CSB-MP354290MVH
CSB-EP354290MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP354291SKY1
CSB-EP354291SKY1
CSB-BP354291SKY1
CSB-MP354291SKY1
CSB-EP354291SKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Uncharacterized protein spr0007 (spr0007) CSB-YP354292SMP
CSB-EP354292SMP
CSB-BP354292SMP
CSB-MP354292SMP
CSB-EP354292SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein htpG (htpG), partial CSB-YP354293MVH
CSB-EP354293MVH
CSB-BP354293MVH
CSB-MP354293MVH
CSB-EP354293MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Imidazolonepropionase (hutI) CSB-YP354294SKY
CSB-EP354294SKY
CSB-BP354294SKY
CSB-MP354294SKY
CSB-EP354294SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein HypA (hypA) CSB-YP354295SWW
CSB-EP354295SWW
CSB-BP354295SWW
CSB-MP354295SWW
CSB-EP354295SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable hydrogenase nickel incorporation protein HybF (hybF) CSB-YP354296SWW
CSB-EP354296SWW
CSB-BP354296SWW
CSB-MP354296SWW
CSB-EP354296SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0748 lipoprotein yddW (yddW) CSB-YP354297ENV
CSB-EP354297ENV
CSB-BP354297ENV
CSB-MP354297ENV
CSB-EP354297ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Inner membrane protein ypjD (ypjD), partial CSB-YP354298ENV
CSB-EP354298ENV
CSB-BP354298ENV
CSB-MP354298ENV
CSB-EP354298ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Inner membrane protein ypjD (ypjD), partial CSB-YP354299EOD
CSB-EP354299EOD
CSB-BP354299EOD
CSB-MP354299EOD
CSB-EP354299EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Uncharacterized protein ybgS (ybgS) CSB-YP354300SXB
CSB-EP354300SXB
CSB-BP354300SXB
CSB-MP354300SXB
CSB-EP354300SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Inner membrane protein ybjM (ybjM), partial CSB-YP354301ENV1
CSB-EP354301ENV1
CSB-BP354301ENV1
CSB-MP354301ENV1
CSB-EP354301ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yceO (yceO) CSB-YP354302SZB
CSB-EP354302SZB
CSB-BP354302SZB
CSB-MP354302SZB
CSB-EP354302SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein ynaJ (ynaJ), partial CSB-YP354303EGX1
CSB-EP354303EGX1
CSB-BP354303EGX1
CSB-MP354303EGX1
CSB-EP354303EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized lipoprotein ydcL (ydcL) CSB-YP354304ENV
CSB-EP354304ENV
CSB-BP354304ENV
CSB-MP354304ENV
CSB-EP354304ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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