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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Trigger factor (tig) CSB-YP354242SWW
CSB-EP354242SWW
CSB-BP354242SWW
CSB-MP354242SWW
CSB-EP354242SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA gyrase subunit B (gyrB), partial CSB-YP354243SKY
CSB-EP354243SKY
CSB-BP354243SKY
CSB-MP354243SKY
CSB-EP354243SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable acetolactate synthase (ilvG), partial CSB-YP354244MVH
CSB-EP354244MVH
CSB-BP354244MVH
CSB-MP354244MVH
CSB-EP354244MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium TPR repeat-containing protein yfgC (yfgC) CSB-YP354245SXB
CSB-EP354245SXB
CSB-BP354245SXB
CSB-MP354245SXB
CSB-EP354245SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable thiol peroxidase (tpx) CSB-YP354246SKX
CSB-EP354246SKX
CSB-BP354246SKX
CSB-MP354246SKX
CSB-EP354246SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transaldolase B (talB) CSB-YP354247SWW
CSB-EP354247SWW
CSB-BP354247SWW
CSB-MP354247SWW
CSB-EP354247SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative peptide transport permease protein Rv1283c/MT1320 (Rv1283c, MT1320), partial CSB-YP354248MVZ1
CSB-EP354248MVZ1
CSB-BP354248MVZ1
CSB-MP354248MVZ1
CSB-EP354248MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus tRNA (guanine-N (1)-)-methyltransferase CSB-YP354249SKY
CSB-EP354249SKY
CSB-BP354249SKY
CSB-MP354249SKY
CSB-EP354249SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP354251SMI
CSB-EP354251SMI
CSB-BP354251SMI
CSB-MP354251SMI
CSB-EP354251SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tryptophan synthase alpha chain (trpA) CSB-YP354252SKY
CSB-EP354252SKY
CSB-BP354252SKY
CSB-MP354252SKY
CSB-EP354252SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tryptophan synthase beta chain (trpB) CSB-YP354253SKY
CSB-EP354253SKY
CSB-BP354253SKY
CSB-MP354253SKY
CSB-EP354253SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Indole-3-glycerol phosphate synthase (trpC) CSB-YP354254SKY
CSB-EP354254SKY
CSB-BP354254SKY
CSB-MP354254SKY
CSB-EP354254SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Anthranilate phosphoribosyltransferase (trpD) CSB-YP354255NGG
CSB-EP354255NGG
CSB-BP354255NGG
CSB-MP354255NGG
CSB-EP354255NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthranilate phosphoribosyltransferase (trpD) CSB-YP354256FMW
CSB-EP354256FMW
CSB-BP354256FMW
CSB-MP354256FMW
CSB-EP354256FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Alanine--tRNA ligase (alaS), partial CSB-YP354259SKY
CSB-EP354259SKY
CSB-BP354259SKY
CSB-MP354259SKY
CSB-EP354259SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Aspartate--tRNA ligase (aspS), partial CSB-YP354260SKY
CSB-EP354260SKY
CSB-BP354260SKY
CSB-MP354260SKY
CSB-EP354260SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glutamate--tRNA ligase (gltX) CSB-YP354261SKX
CSB-EP354261SKX
CSB-BP354261SKX
CSB-MP354261SKX
CSB-EP354261SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP354262EGX
CSB-EP354262EGX
CSB-BP354262EGX
CSB-MP354262EGX
CSB-EP354262EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP354263NGG
CSB-EP354263NGG
CSB-BP354263NGG
CSB-MP354263NGG
CSB-EP354263NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine--tRNA ligase (glyQS) CSB-YP354264MVZ
CSB-EP354264MVZ
CSB-BP354264MVZ
CSB-MP354264MVZ
CSB-EP354264MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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