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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ferredoxin-1 CSB-YP355498AMC
CSB-EP355498AMC
CSB-BP355498AMC
CSB-MP355498AMC
CSB-EP355498AMC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin-1 CSB-YP355499NEG
CSB-EP355499NEG
CSB-BP355499NEG
CSB-MP355499NEG
CSB-EP355499NEG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marichromatium gracile High-potential iron-sulfur protein (hip) CSB-YP355500MCC
CSB-EP355500MCC
CSB-BP355500MCC
CSB-MP355500MCC
CSB-EP355500MCC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus sp. High-potential iron-sulfur protein (hip) CSB-YP355501PCB
CSB-EP355501PCB
CSB-BP355501PCB
CSB-MP355501PCB
CSB-EP355501PCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptostreptococcus asaccharolyticus Rubredoxin CSB-YP355502PFO
CSB-EP355502PFO
CSB-BP355502PFO
CSB-MP355502PFO
CSB-EP355502PFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rubredoxin CSB-YP355503MQR
CSB-EP355503MQR
CSB-BP355503MQR
CSB-MP355503MQR
CSB-EP355503MQR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Glutaredoxin (NRDC) CSB-YP355504EDZ
CSB-EP355504EDZ
CSB-BP355504EDZ
CSB-MP355504EDZ
CSB-EP355504EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azurin CSB-YP355505PUI
CSB-EP355505PUI
CSB-BP355505PUI
CSB-MP355505PUI
CSB-EP355505PUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vicia faba Plastocyanin (PETE) CSB-YP355506VFJ
CSB-EP355506VFJ
CSB-BP355506VFJ
CSB-MP355506VFJ
CSB-EP355506VFJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumis sativus Plastocyanin (PETE) CSB-YP355507CXP
CSB-EP355507CXP
CSB-BP355507CXP
CSB-MP355507CXP
CSB-EP355507CXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum crispum Plastocyanin (PETE) CSB-YP355508SIN
CSB-EP355508SIN
CSB-BP355508SIN
CSB-MP355508SIN
CSB-EP355508SIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumis sativus Basic blue protein CSB-YP355509CXP
CSB-EP355509CXP
CSB-BP355509CXP
CSB-MP355509CXP
CSB-EP355509CXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Galdieria sulphuraria C-phycocyanin alpha chain (cpcA) CSB-YP355510GAD
CSB-EP355510GAD
CSB-BP355510GAD
CSB-MP355510GAD
CSB-EP355510GAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Galdieria sulphuraria C-phycocyanin beta chain (cpcB) CSB-YP355511GAD
CSB-EP355511GAD
CSB-BP355511GAD
CSB-MP355511GAD
CSB-EP355511GAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Allophycocyanin alpha chain (apcA) CSB-YP355512DZX
CSB-EP355512DZX
CSB-BP355512DZX
CSB-MP355512DZX
CSB-EP355512DZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Allophycocyanin beta chain (apcB) CSB-YP355513DZX
CSB-EP355513DZX
CSB-BP355513DZX
CSB-MP355513DZX
CSB-EP355513DZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus L-lactate dehydrogenase (ldh) CSB-YP355514GFM
CSB-EP355514GFM
CSB-BP355514GFM
CSB-MP355514GFM
CSB-EP355514GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Glyceraldehyde-3-phosphate dehydrogenase 1 (TDH1) CSB-YP355515SVG
CSB-EP355515SVG
CSB-BP355515SVG
CSB-MP355515SVG
CSB-EP355515SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa NAD-specific glutamate dehydrogenase (gdh-1), partial CSB-YP355516NHA
CSB-EP355516NHA
CSB-BP355516NHA
CSB-MP355516NHA
CSB-EP355516NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Methylamine dehydrogenase light chain (mauA) CSB-YP355517MSZ
CSB-EP355517MSZ
CSB-BP355517MSZ
CSB-MP355517MSZ
CSB-EP355517MSZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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