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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bovine parainfluenza 3 virus Hemagglutinin-neuraminidase (HN), partial CSB-YP356733BKP1
CSB-EP356733BKP1
CSB-BP356733BKP1
CSB-MP356733BKP1
CSB-EP356733BKP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Pyruvate decarboxylase isozyme 1 (PDC1), partial CSB-YP356734SVG
CSB-EP356734SVG
CSB-BP356734SVG
CSB-MP356734SVG
CSB-EP356734SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Histone-binding protein N1/N2, partial CSB-YP356735XBE
CSB-EP356735XBE
CSB-BP356735XBE
CSB-MP356735XBE
CSB-EP356735XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Phosphoglycerate transport system transcriptional regulatory protein pgtA (pgtA) CSB-YP356736SXB
CSB-EP356736SXB
CSB-BP356736SXB
CSB-MP356736SXB
CSB-EP356736SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Ribulokinase (araB), partial CSB-YP356737SXB
CSB-EP356737SXB
CSB-BP356737SXB
CSB-MP356737SXB
CSB-EP356737SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium L-ribulose-5-phosphate 4-epimerase (araD) CSB-YP356738SXB
CSB-EP356738SXB
CSB-BP356738SXB
CSB-MP356738SXB
CSB-EP356738SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Para-aminobenzoate synthase glutamine amidotransferase component II (pabA) CSB-YP356739SXB
CSB-EP356739SXB
CSB-BP356739SXB
CSB-MP356739SXB
CSB-EP356739SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Para-aminobenzoate synthase glutamine amidotransferase component II (pabA) CSB-YP356740SYN
CSB-EP356740SYN
CSB-BP356740SYN
CSB-MP356740SYN
CSB-EP356740SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Periplasmic oligopeptide-binding protein (oppA), partial CSB-YP356741SXB
CSB-EP356741SXB
CSB-BP356741SXB
CSB-MP356741SXB
CSB-EP356741SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Dihydroorotase (pyrC) CSB-YP356742SXB
CSB-EP356742SXB
CSB-BP356742SXB
CSB-MP356742SXB
CSB-EP356742SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 2-isopropylmalate synthase (LEU4), partial CSB-YP356743SVG
CSB-EP356743SVG
CSB-BP356743SVG
CSB-MP356743SVG
CSB-EP356743SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Poliovirus type 2 Genome polyprotein CSB-YP356744POC
CSB-EP356744POC
CSB-BP356744POC
CSB-MP356744POC
CSB-EP356744POC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogen regulation protein ntrB (ntrB) CSB-YP356745KBE
CSB-EP356745KBE
CSB-BP356745KBE
CSB-MP356745KBE
CSB-EP356745KBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Lactose operon repressor (lacI) CSB-YP356746KBG
CSB-EP356746KBG
CSB-BP356746KBG
CSB-MP356746KBG
CSB-EP356746KBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA polymerase sigma-54 factor (rpoN) CSB-YP356747KBE
CSB-EP356747KBE
CSB-BP356747KBE
CSB-MP356747KBE
CSB-EP356747KBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage SP02 DNA polymerase (L), partial CSB-YP356748BBI
CSB-EP356748BBI
CSB-BP356748BBI
CSB-MP356748BBI
CSB-EP356748BBI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage SP01 RNA polymerase sigma GP34 factor (34) CSB-YP356749BQY
CSB-EP356749BQY
CSB-BP356749BQY
CSB-MP356749BQY
CSB-EP356749BQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T5 Exodeoxyribonuclease (D15) CSB-YP356750ELS
CSB-EP356750ELS
CSB-BP356750ELS
CSB-MP356750ELS
CSB-EP356750ELS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Nodulation protein E (nodE), partial CSB-YP356751RKT1
CSB-EP356751RKT1
CSB-BP356751RKT1
CSB-MP356751RKT1
CSB-EP356751RKT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Nodulation protein F (nodF) CSB-YP356752RKT
CSB-EP356752RKT
CSB-BP356752RKT
CSB-MP356752RKT
CSB-EP356752RKT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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