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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Tubulin alpha-3 chain (TUB3) CSB-YP357747SVG
CSB-EP357747SVG
CSB-BP357747SVG
CSB-MP357747SVG
CSB-EP357747SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halobacterium salinarum Superoxide dismutase [Mn] 1 (sod1) CSB-YP357748HTM
CSB-EP357748HTM
CSB-BP357748HTM
CSB-MP357748HTM
CSB-EP357748HTM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Troponin T, fast skeletal muscle (Tnnt3) CSB-YP357749RA
CSB-EP357749RA
CSB-BP357749RA
CSB-MP357749RA
CSB-EP357749RA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shufflon protein A CSB-YP357750ENL
CSB-EP357750ENL
CSB-BP357750ENL
CSB-MP357750ENL
CSB-EP357750ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shufflon protein B CSB-YP357751ENL
CSB-EP357751ENL
CSB-BP357751ENL
CSB-MP357751ENL
CSB-EP357751ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shufflon protein C CSB-YP357752ENL
CSB-EP357752ENL
CSB-BP357752ENL
CSB-MP357752ENL
CSB-EP357752ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas moewusii Uncharacterized 38.5 kDa protein in psbA intron 1 CSB-YP357754CIX
CSB-EP357754CIX
CSB-BP357754CIX
CSB-MP357754CIX
CSB-EP357754CIX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max Chlorophyll a-b binding protein 2, chloroplastic (CAB2), partial CSB-YP357755GGV1
CSB-EP357755GGV1
CSB-BP357755GGV1
CSB-MP357755GGV1
CSB-EP357755GGV1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ureaplasma urealyticum Tetracycline resistance protein tetM (tetM), partial CSB-YP357756UAP
CSB-EP357756UAP
CSB-BP357756UAP
CSB-MP357756UAP
CSB-EP357756UAP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Wound-induced protein WIN1 (WIN1) CSB-YP357757FIG
CSB-EP357757FIG
CSB-BP357757FIG
CSB-MP357757FIG
CSB-EP357757FIG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L11 (rplK) CSB-YP357758SYN
CSB-EP357758SYN
CSB-BP357758SYN
CSB-MP357758SYN
CSB-EP357758SYN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Hemagglutinin (HA), partial CSB-YP357759IJO
CSB-EP357759IJO
CSB-BP357759IJO
CSB-MP357759IJO
CSB-EP357759IJO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Hemagglutinin (HA), partial CSB-YP357760IJY
CSB-EP357760IJY
CSB-BP357760IJY
CSB-MP357760IJY
CSB-EP357760IJY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Ornithine cyclodeaminase (arcB) CSB-YP357761AYS
CSB-EP357761AYS
CSB-BP357761AYS
CSB-MP357761AYS
CSB-EP357761AYS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Achaete-scute complex protein T8 (ase) CSB-YP357762DLU
CSB-EP357762DLU
CSB-BP357762DLU
CSB-MP357762DLU
CSB-EP357762DLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium radiobacter Protein virB6 (virB6), partial CSB-YP357763RKV1
CSB-EP357763RKV1
CSB-BP357763RKV1
CSB-MP357763RKV1
CSB-EP357763RKV1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium radiobacter Protein virB10 (virB10), partial CSB-YP357764RKV1
CSB-EP357764RKV1
CSB-BP357764RKV1
CSB-MP357764RKV1
CSB-EP357764RKV1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Anthranilate synthase component II, pyocyanin specific (phnB) CSB-YP357765EZX
CSB-EP357765EZX
CSB-BP357765EZX
CSB-MP357765EZX
CSB-EP357765EZX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 4-cresol dehydrogenase [hydroxylating] flavoprotein subunit (pchF), partial CSB-YP357766FFZ
CSB-EP357766FFZ
CSB-BP357766FFZ
CSB-MP357766FFZ
CSB-EP357766FFZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae IgA-specific serine endopeptidase autotransporter (iga), partial CSB-YP357767NFZ
CSB-EP357767NFZ
CSB-BP357767NFZ
CSB-MP357767NFZ
CSB-EP357767NFZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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