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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cryptococcus albidus Endo-1,4-beta-xylanase CSB-YP357136CTI
CSB-EP357136CTI
CSB-BP357136CTI
CSB-MP357136CTI
CSB-EP357136CTI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage PZA Major head protein (8) CSB-YP357137BBE
CSB-EP357137BBE
CSB-BP357137BBE
CSB-MP357137BBE
CSB-EP357137BBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage PZA Head morphogenesis protein (7) CSB-YP357138BBE
CSB-EP357138BBE
CSB-BP357138BBE
CSB-MP357138BBE
CSB-EP357138BBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage PZA Upper collar protein (10) CSB-YP357139BBE
CSB-EP357139BBE
CSB-BP357139BBE
CSB-MP357139BBE
CSB-EP357139BBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage PZA Morphogenesis protein 1 (13) CSB-YP357140BBE
CSB-EP357140BBE
CSB-BP357140BBE
CSB-MP357140BBE
CSB-EP357140BBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage PZA Lysozyme (15) CSB-YP357141BBE
CSB-EP357141BBE
CSB-BP357141BBE
CSB-MP357141BBE
CSB-EP357141BBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium radiobacter Protein virE1 (virE1) CSB-YP357142RKV
CSB-EP357142RKV
CSB-BP357142RKV
CSB-MP357142RKV
CSB-EP357142RKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Pentafunctional AROM polypeptide (aromA), partial CSB-YP357143EKF
CSB-EP357143EKF
CSB-BP357143EKF
CSB-MP357143EKF
CSB-EP357143EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mastigocladus laminosus Phycobiliprotein beta chain (apcD) CSB-YP357144MQJ
CSB-EP357144MQJ
CSB-BP357144MQJ
CSB-MP357144MQJ
CSB-EP357144MQJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Tetracycline resistance protein (tet), partial CSB-YP357145GFM
CSB-EP357145GFM
CSB-BP357145GFM
CSB-MP357145GFM
CSB-EP357145GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dengue virus type 2 Genome polyprotein, partial CSB-YP357146DCD
CSB-EP357146DCD
CSB-BP357146DCD
CSB-MP357146DCD
CSB-EP357146DCD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mason-Pfizer monkey virus Gag polyprotein (gag) CSB-YP357147MCJ
CSB-EP357147MCJ
CSB-BP357147MCJ
CSB-MP357147MCJ
CSB-EP357147MCJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mason-Pfizer monkey virus Pol polyprotein (pol), partial CSB-YP357148MCJ
CSB-EP357148MCJ
CSB-BP357148MCJ
CSB-MP357148MCJ
CSB-EP357148MCJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian virus 5 Small hydrophobic protein (SH), partial CSB-YP357149SHU
CSB-EP357149SHU
CSB-BP357149SHU
CSB-MP357149SHU
CSB-EP357149SHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas phage phi6 Morphogenetic protein (P12) CSB-YP357150PUV
CSB-EP357150PUV
CSB-BP357150PUV
CSB-MP357150PUV
CSB-EP357150PUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas phage phi6 Phage lysis protein (P5) CSB-YP357151PUV
CSB-EP357151PUV
CSB-BP357151PUV
CSB-MP357151PUV
CSB-EP357151PUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Astacus fluviatilis Astacin CSB-YP357152APX
CSB-EP357152APX
CSB-BP357152APX
CSB-MP357152APX
CSB-EP357152APX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heat-stable enterotoxin A (ystA) CSB-YP357153YAQ
CSB-EP357153YAQ
CSB-BP357153YAQ
CSB-MP357153YAQ
CSB-EP357153YAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Non-specific lipid-transfer protein 1 (LTP1) CSB-YP357154HWQ
CSB-EP357154HWQ
CSB-BP357154HWQ
CSB-MP357154HWQ
CSB-EP357154HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Tryptophan synthase beta chain (trpB) CSB-YP357155BRJ
CSB-EP357155BRJ
CSB-BP357155BRJ
CSB-MP357155BRJ
CSB-EP357155BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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