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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Profilin (PFY1) CSB-YP357075SVG
CSB-EP357075SVG
CSB-BP357075SVG
CSB-MP357075SVG
CSB-EP357075SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA repair protein RAD2 (RAD2), partial CSB-YP357076SVG
CSB-EP357076SVG
CSB-BP357076SVG
CSB-MP357076SVG
CSB-EP357076SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae cAMP-dependent protein kinase regulatory subunit (BCY1) CSB-YP357077SVG
CSB-EP357077SVG
CSB-BP357077SVG
CSB-MP357077SVG
CSB-EP357077SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 40S ribosomal protein S19-B (RPS19B) CSB-YP357078SVG
CSB-EP357078SVG
CSB-BP357078SVG
CSB-MP357078SVG
CSB-EP357078SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aequipecten irradians Myosin essential light chain, striated adductor muscle CSB-YP357080ADN
CSB-EP357080ADN
CSB-BP357080ADN
CSB-MP357080ADN
CSB-EP357080ADN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Relaxosome protein TraM (traM) CSB-YP357081ENL
CSB-EP357081ENL
CSB-BP357081ENL
CSB-MP357081ENL
CSB-EP357081ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine parvovirus Non-capsid protein NS-1 (NS1), partial CSB-YP357082BKQ
CSB-EP357082BKQ
CSB-BP357082BKQ
CSB-MP357082BKQ
CSB-EP357082BKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human parvovirus B19 Non-capsid protein NS-1 (NS1), partial CSB-YP357083HPM
CSB-BP357083HPM
CSB-MP357083HPM
CSB-EP357083HPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine minute virus Non-capsid protein NS-1 (NS1), partial CSB-YP357084MKF
CSB-EP357084MKF
CSB-BP357084MKF
CSB-MP357084MKF
CSB-EP357084MKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tubulin alpha-1 chain CSB-YP357085SQX
CSB-EP357085SQX
CSB-BP357085SQX
CSB-MP357085SQX
CSB-EP357085SQX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Acyl-CoA desaturase 1 (Scd1), partial CSB-YP357086RA1
CSB-EP357086RA1
CSB-BP357086RA1
CSB-MP357086RA1
CSB-EP357086RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Allophycocyanin alpha chain (apcA) CSB-YP357087AJK
CSB-EP357087AJK
CSB-BP357087AJK
CSB-MP357087AJK
CSB-EP357087AJK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase molybdenum-iron protein beta chain (nifK), partial CSB-YP357088DPX
CSB-EP357088DPX
CSB-BP357088DPX
CSB-MP357088DPX
CSB-EP357088DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Moloney murine sarcoma virus Serine/threonine-protein kinase-transforming protein mos (V-MOS) CSB-YP357089MHM
CSB-EP357089MHM
CSB-BP357089MHM
CSB-MP357089MHM
CSB-EP357089MHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Tryptophan synthase beta chain (trpB) CSB-YP357090EZX
CSB-EP357090EZX
CSB-BP357090EZX
CSB-MP357090EZX
CSB-EP357090EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae N-terminal acetyltransferase A complex catalytic subunit ARD1 (ARD1) CSB-YP357091SVG
CSB-EP357091SVG
CSB-BP357091SVG
CSB-MP357091SVG
CSB-EP357091SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium DNA primase (dnaG), partial CSB-YP357092SXB
CSB-EP357092SXB
CSB-BP357092SXB
CSB-MP357092SXB
CSB-EP357092SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Chemotaxis protein methyltransferase (cheR) CSB-YP357093ENV
CSB-EP357093ENV
CSB-BP357093ENV
CSB-MP357093ENV
CSB-EP357093ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Light-harvesting protein B-800/850 beta chain (pucB), partial CSB-YP357094RLC
CSB-EP357094RLC
CSB-BP357094RLC
CSB-MP357094RLC
CSB-EP357094RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Chlorophyll a-b binding protein 1B, chloroplastic (CAB1B), partial CSB-YP357095SZY1
CSB-EP357095SZY1
CSB-BP357095SZY1
CSB-MP357095SZY1
CSB-EP357095SZY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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