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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Outer membrane protein H.8 CSB-YP357055NFZ
CSB-EP357055NFZ
CSB-BP357055NFZ
CSB-MP357055NFZ
CSB-EP357055NFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pleuronectes platessa Metallothionein (mt) CSB-YP357056PNG
CSB-EP357056PNG
CSB-BP357056PNG
CSB-MP357056PNG
CSB-EP357056PNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris Phenylalanine ammonia-lyase class 1, partial CSB-YP357057EUN
CSB-EP357057EUN
CSB-BP357057EUN
CSB-MP357057EUN
CSB-EP357057EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lymnaea stagnalis Molluscan insulin-related peptide 1 CSB-YP357058LRQ
CSB-EP357058LRQ
CSB-BP357058LRQ
CSB-MP357058LRQ
CSB-EP357058LRQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lithobates catesbeiana Ferritin, higher subunit CSB-YP357059LQA
CSB-EP357059LQA
CSB-BP357059LQA
CSB-MP357059LQA
CSB-EP357059LQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage GA Lysis protein CSB-YP357060ECM
CSB-EP357060ECM
CSB-BP357060ECM
CSB-MP357060ECM
CSB-EP357060ECM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage GA Coat protein CSB-YP357061ECM
CSB-EP357061ECM
CSB-BP357061ECM
CSB-MP357061ECM
CSB-EP357061ECM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Dual specificity protein phosphatase H1 (H1L) CSB-YP357062VAI
CSB-EP357062VAI
CSB-BP357062VAI
CSB-MP357062VAI
CSB-EP357062VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Bifunctional purine biosynthetic protein ADE5,7 (ADE5,7), partial CSB-YP357063SVG
CSB-EP357063SVG
CSB-BP357063SVG
CSB-MP357063SVG
CSB-EP357063SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Regulatory protein ADR1 (ADR1), partial CSB-YP357064SVG
CSB-EP357064SVG
CSB-BP357064SVG
CSB-MP357064SVG
CSB-EP357064SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cytochrome B pre-mRNA-processing protein 1 (CBP1), partial CSB-YP357065SVG
CSB-EP357065SVG
CSB-BP357065SVG
CSB-MP357065SVG
CSB-EP357065SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chitinase A (chiA), partial CSB-YP357066SYN
CSB-EP357066SYN
CSB-BP357066SYN
CSB-MP357066SYN
CSB-EP357066SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cytochrome b-c1 complex subunit 1, mitochondrial (COR1) CSB-YP357067SVG
CSB-EP357067SVG
CSB-BP357067SVG
CSB-MP357067SVG
CSB-EP357067SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Carbamoyl-phosphate synthase arginine-specific small chain (CPA1) CSB-YP357068SVG
CSB-EP357068SVG
CSB-BP357068SVG
CSB-MP357068SVG
CSB-EP357068SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae NADP-specific glutamate dehydrogenase 1 (GDH1) CSB-YP357069SVG
CSB-EP357069SVG
CSB-BP357069SVG
CSB-MP357069SVG
CSB-EP357069SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 3-isopropylmalate dehydratase (LEU1), partial CSB-YP357070SVG
CSB-EP357070SVG
CSB-BP357070SVG
CSB-MP357070SVG
CSB-EP357070SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial RNA-splicing protein MRS1 (MRS1) CSB-YP357071SVG
CSB-EP357071SVG
CSB-BP357071SVG
CSB-MP357071SVG
CSB-EP357071SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia marcescens Serralysin CSB-YP357072SFK
CSB-EP357072SFK
CSB-BP357072SFK
CSB-MP357072SFK
CSB-EP357072SFK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Phosphate system positive regulatory protein PHO4 (PHO4) CSB-YP357073SVG
CSB-EP357073SVG
CSB-BP357073SVG
CSB-MP357073SVG
CSB-EP357073SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Pyrimidine pathway regulatory protein 1 (PPR1), partial CSB-YP357074SVG
CSB-EP357074SVG
CSB-BP357074SVG
CSB-MP357074SVG
CSB-EP357074SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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