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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chlamydomonas reinhardtii Ribulose bisphosphate carboxylase small chain 2, chloroplastic (RBCS-2) CSB-YP357402DSJ
CSB-EP357402DSJ
CSB-BP357402DSJ
CSB-MP357402DSJ
CSB-EP357402DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Glyceraldehyde-3-phosphate dehydrogenase, cytosolic (GAPC) CSB-YP357403HWQ
CSB-EP357403HWQ
CSB-BP357403HWQ
CSB-MP357403HWQ
CSB-EP357403HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vulpes vulpes Double-headed protease inhibitor, submandibular gland CSB-YP357404VFW
CSB-EP357404VFW
CSB-BP357404VFW
CSB-MP357404VFW
CSB-EP357404VFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Panthera leo Double-headed protease inhibitor, submandibular gland CSB-YP357405PBD
CSB-EP357405PBD
CSB-BP357405PBD
CSB-MP357405PBD
CSB-EP357405PBD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Glutenin, high molecular weight subunit 12, partial CSB-YP357406TQN
CSB-EP357406TQN
CSB-BP357406TQN
CSB-MP357406TQN
CSB-EP357406TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Glutenin, high molecular weight subunit PW212, partial CSB-YP357407TQN
CSB-EP357407TQN
CSB-BP357407TQN
CSB-MP357407TQN
CSB-EP357407TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ross river virus Structural polyprotein, partial CSB-YP357408RFM1
CSB-EP357408RFM1
CSB-BP357408RFM1
CSB-MP357408RFM1
CSB-EP357408RFM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Aspartokinase 2 (lysC) CSB-YP357409BRJ
CSB-EP357409BRJ
CSB-BP357409BRJ
CSB-MP357409BRJ
CSB-EP357409BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Homoserine dehydrogenase (hom) CSB-YP357410DWY
CSB-EP357410DWY
CSB-BP357410DWY
CSB-MP357410DWY
CSB-EP357410DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposase for transposon Tn2501 (tnpA), partial CSB-YP357411ENL
CSB-EP357411ENL
CSB-BP357411ENL
CSB-MP357411ENL
CSB-EP357411ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Low affinity immunoglobulin gamma Fc region receptor III (Fcgr3), partial CSB-YP357412MO
CSB-BP357412MO
CSB-EP357412MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Potassium voltage-gated channel protein Shaker (Sh), partial CSB-YP357413DLU1
CSB-EP357413DLU1
CSB-BP357413DLU1
CSB-MP357413DLU1
CSB-EP357413DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Electrophorus electricus Beta-galactoside-binding lectin CSB-YP357415EJY
CSB-EP357415EJY
CSB-BP357415EJY
CSB-MP357415EJY
CSB-EP357415EJY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP357416ZAX
CSB-EP357416ZAX
CSB-BP357416ZAX
CSB-MP357416ZAX
CSB-EP357416ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays 30S ribosomal protein S8, chloroplastic (rps8) CSB-YP357417ZAX
CSB-EP357417ZAX
CSB-BP357417ZAX
CSB-MP357417ZAX
CSB-EP357417ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Sulfate adenylyltransferase (MET3), partial CSB-YP357418SVG
CSB-EP357418SVG
CSB-BP357418SVG
CSB-MP357418SVG
CSB-EP357418SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyrosine-protein phosphatase yopH (yopH) CSB-YP357419YAH
CSB-EP357419YAH
CSB-BP357419YAH
CSB-MP357419YAH
CSB-EP357419YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus DNA polymerase catalytic subunit (UL54), partial CSB-YP357421HWV
CSB-EP357421HWV
CSB-BP357421HWV
CSB-MP357421HWV
CSB-EP357421HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine coronavirus Membrane protein (M), partial CSB-YP357422MKA1
CSB-EP357422MKA1
CSB-BP357422MKA1
CSB-MP357422MKA1
CSB-EP357422MKA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage Mu Baseplate protein (P) CSB-YP357423EDA
CSB-EP357423EDA
CSB-BP357423EDA
CSB-MP357423EDA
CSB-EP357423EDA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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