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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human cytomegalovirus Glycoprotein UL18 (H301), partial CSB-YP357424HWV1
CSB-EP357424HWV1
CSB-BP357424HWV1
CSB-MP357424HWV1
CSB-EP357424HWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rubella virus Structural polyprotein, partial CSB-YP357425RHR1
CSB-EP357425RHR1
CSB-BP357425RHR1
CSB-MP357425RHR1
CSB-EP357425RHR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Pentafunctional AROM polypeptide (ARO1), partial CSB-YP357426SVG
CSB-EP357426SVG
CSB-BP357426SVG
CSB-MP357426SVG
CSB-EP357426SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Submandibular gland secretory Glx-rich protein CA (Grpca) CSB-YP357427RA
CSB-EP357427RA
CSB-BP357427RA
CSB-MP357427RA
CSB-EP357427RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Ribosomal protein S16, mitochondrial (cyt-21) CSB-YP357428NHA
CSB-EP357428NHA
CSB-BP357428NHA
CSB-MP357428NHA
CSB-EP357428NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Late protein H7 (VACWR105), partial CSB-YP357429VAI1
CSB-EP357429VAI1
CSB-BP357429VAI1
CSB-MP357429VAI1
CSB-EP357429VAI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein MSS18 (MSS18) CSB-YP357430SVG
CSB-EP357430SVG
CSB-BP357430SVG
CSB-MP357430SVG
CSB-EP357430SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scyliorhinus canicula Scyliorhinin-1 CSB-YP357431SYA
CSB-EP357431SYA
CSB-BP357431SYA
CSB-MP357431SYA
CSB-EP357431SYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phyllomedusa bicolor Phyllomedusin CSB-YP357432PIP
CSB-EP357432PIP
CSB-BP357432PIP
CSB-MP357432PIP
CSB-EP357432PIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uperoleia rugosa Uperolein CSB-YP357433UAL
CSB-EP357433UAL
CSB-BP357433UAL
CSB-MP357433UAL
CSB-EP357433UAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kassina maculata Hylambatin CSB-YP357434KBA
CSB-EP357434KBA
CSB-BP357434KBA
CSB-MP357434KBA
CSB-EP357434KBA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uperoleia rugosa Rugosauperolein-2 CSB-YP357435UAL
CSB-EP357435UAL
CSB-BP357435UAL
CSB-MP357435UAL
CSB-EP357435UAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Nitrate reductase [NADPH] (nit-3), partial CSB-YP357436NHA
CSB-EP357436NHA
CSB-BP357436NHA
CSB-MP357436NHA
CSB-EP357436NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA polymerase sigma-54 factor (rpoN), partial CSB-YP357437DPX
CSB-EP357437DPX
CSB-BP357437DPX
CSB-MP357437DPX
CSB-EP357437DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase iron protein 2 (nifH2) CSB-YP357438MSC
CSB-EP357438MSC
CSB-BP357438MSC
CSB-MP357438MSC
CSB-EP357438MSC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nodulation protein L (nodL) CSB-YP357439RKR
CSB-EP357439RKR
CSB-BP357439RKR
CSB-MP357439RKR
CSB-EP357439RKR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nodulation protein N (nodN) CSB-YP357440RKR
CSB-EP357440RKR
CSB-BP357440RKR
CSB-MP357440RKR
CSB-EP357440RKR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Regulatory protein LEU3 (LEU3), partial CSB-YP357441SVG
CSB-EP357441SVG
CSB-BP357441SVG
CSB-MP357441SVG
CSB-EP357441SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Flocculation protein FLO11 (MUC1), partial CSB-YP357442SVG
CSB-EP357442SVG
CSB-BP357442SVG
CSB-MP357442SVG
CSB-EP357442SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken GTPase HRas (HRAS1) CSB-YP357443CH
CSB-EP357443CH
CSB-BP357443CH
CSB-MP357443CH
CSB-EP357443CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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